汉黄芩素抑制乳腺癌细胞增殖及侵袭的实验研究

目的：研究汉黄芩素对乳腺癌细胞MDA-MB-231生长和增殖的影响，同时观察汉黄芩素对MDA-MB-231细胞黏附、迁移和侵袭能力的影响，进一步探讨其分子作用机制。方法：MTT法检测汉黄芩素对MDA-MB-231细胞生长的影响；Ki-67法检测汉黄芩素对细胞增殖的影响；划痕实验、黏附试验和侵袭小室法检测对MDA-MB-231细胞迁移和侵袭能力的影响；Western blotting检测对增殖和转移相关蛋白及相关信号通路的影响。结果：汉黄芩素能明显抑制MDA-MB-231细胞生长和增殖；低浓度情况下明显抑制乳腺癌细胞的迁移、黏附和侵袭能力；汉黄芩素有效抑制MDA-MB-231细胞Survivin，Bcl-2，ICAM-1，MMP-2，MMP-9蛋白的表达。结论：汉黄芩素明显抑制乳腺癌细胞生长和增殖，并抑制MDA-MB-231细胞迁移、黏附、侵袭，其对MDA-MB-231细胞的侵袭和黏附影响可能与其降低ICAM-1，MMP-2，MMP-9表达有关。

Experimental study on inhibitory effect of wogonin on proliferation and invasion of breast cancer cells

Objective: To study the inhibitory effect of wogonin on the growth and proliferation of breast cancer cells MDA-MB-23, and observe its effect on the adhesion, migration and invasion of MDA-MB-23 cells, in order to further study its molecular mechanism. Method: MTT assay was used to detect the effect of wogonin on MDA-MB-23 cell growth. Ki-67 assay was adopted to test the effect of wogonin on cell proliferation. Scratch test, adherence test and invasion chamber assay were taken to detect the effect on the migration and invasion abilities of MDA-MB-231 cells. Proliferation and metastasis-related proteins and relevant signaling pathways were detected by Western blotting. Result: Wogonin could remarkably inhibit the growth and proliferation of MDA-MB-231 cells, significantly inhibit migration, adhesion and invasion abilities of breast cancer cells at a low concentration, and effectively inhibit the expression of Survivin, Bcl-2, ICAM-1, MMP-2, MMP-9 proteins of MDA-MB-231 cells. Conclusion: Wogonin could notably inhibit growth and proliferation of breast cancer cells, and inhibit migration, adhesion and invasion of MDA-MB-231 cells. Its invasive and adhesive effects on MDA-MB-231 cells may be related to the decrease in ICAM-1, MMP-2, MMP-9 expressions.