|
|||||
|
|
| 川楝子中肝毒性成分的快速筛查研究 | |
| 引用本文： | 赵筱萍,葛志伟,张玉峰,兰小红,张伯礼. 川楝子中肝毒性成分的快速筛查研究[J]. 中国中药杂志, 2013, 38(11): 1820-1822 |
| 作者姓名： | 赵筱萍 葛志伟 张玉峰 兰小红 张伯礼 |
| 作者单位： | 1. 天津中医药大学,天津300193;浙江中医药大学,浙江杭州310053 2. 浙江大学药学院,浙江杭州,310058 3. 浙江中医药大学,浙江杭州,310053 4. 天津中医药大学,天津,300193 |
| 基金项目： | 国家"重大新药创制" 科技重大专项(2011ZX09307-002-01);浙江省科技计划项目(2008C23065) |
| 摘 要： | 使用荧光探针FDA标记的HepG2细胞模型及细胞荧光显微图像自动分析法,对川楝子的23个化学组分进行快速筛查,发现5个组分具有明显毒性.对其中的2个组分进行液质联用分析,推测鉴定了10个化学成分,制备并鉴定出其中3个成分的分子结构(meliasenin B,trichilinin D,1-O-tigloy-l-O-debenzoylohchinal).进一步实验研究发现,这3个成分对HepG2细胞呈量-毒关系,提示川楝子中这些成分可能引起肝毒性. |
| 关 键 词： | 川楝子 HepG2细胞 中药毒性成分 毒性成分快速筛查 肝毒性 |
| 收稿时间： | 2013-04-06 |
Study on rapid screening and identifying hepatotoxic compounds in Toosendan Fructus |
|
| ZHAO Xiao-ping,GE Zhi-wei,ZHANG Yu-feng,LAN Xiao-hong and ZHANG Bo-li. Study on rapid screening and identifying hepatotoxic compounds in Toosendan Fructus[J]. China Journal of Chinese Materia Medica, 2013, 38(11): 1820-1822 | |
| Authors: | ZHAO Xiao-ping GE Zhi-wei ZHANG Yu-feng LAN Xiao-hong ZHANG Bo-li |
| Affiliation: | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Zhejiang Chinese Medical University, Hangzhou 310053, China;College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China;Zhejiang Chinese Medical University, Hangzhou 310053, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
| Abstract: | Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MSn, and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity. |
| Keywords: | Toosendan Fructus HepG2 cells toxic compounds in traditional Chinese medicine fast screening of toxic compounds hepatotoxicity |
| 本文献已被 万方数据 等数据库收录！ | |