| 信宜润楠的化学成分研究 |
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| 引用本文： | 程伟,朱承根,林生,杨永春,陈晓光,王文杰,石建功.信宜润楠的化学成分研究[J].中国中药杂志,2012,37(15):2289-2295. |
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| 作者姓名： | 程伟 朱承根 林生 杨永春 陈晓光 王文杰 石建功 |
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| 作者单位： | 中国医学科学院北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验室,北京100050 |
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| 基金项目： | 国家自然科学基金项目(30825044,20932007);国家"重大新药创制"科技重大专项(2011ZX09307-002-01) |
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| 摘 要： | 通过萃取、正相硅胶、Sephadex LH-20、闪式柱色谱以及反相HPLC柱色谱等多种分离方法相结合,从信宜润楠乙醇提物中首次分离得到21个化合物;借助红外、质谱和核磁共振等波谱学分析方法鉴定了它们的结构,其中包括8个丁内酯类(1～8),8个木脂素类(9～16)和5个萜类化合物(17～21),化合物16是降七碳木脂烷类新天然产物。经体外活性筛选发现化合物5对胃癌(BGC-823)和卵巢癌(A2780)人肿瘤细胞株有选择性抑制活性,IC50分别为0.13×10-6,2.66×10-6mol.L-1;在1×10-5mol.L-1时,化合物8和9具有明显抑制PAF刺激大鼠多形核白细胞β-葡萄糖苷酸酶释放作用,抑制率分别为60.0%,54.2%。
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| 关 键 词： | 信宜润楠 丁内酯 木脂素 萜类 抑制胃癌(BGC-823)和卵巢癌(A2780)人肿瘤细胞活性 抑制β-葡萄糖苷酸酶释放 |
| 收稿时间： | 2012/4/15 0:00:00 |
Chemical constituents from Machilus wangchiana |
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| CHENG Wei,ZHU Chenggen,LIN Sheng,YANG Yongchun,CHEN Xiaoguang,WANG Wenjie and SHI Jiangong.Chemical constituents from Machilus wangchiana[J].China Journal of Chinese Materia Medica,2012,37(15):2289-2295. |
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| Authors: | CHENG Wei ZHU Chenggen LIN Sheng YANG Yongchun CHEN Xiaoguang WANG Wenjie and SHI Jiangong |
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| Institution: | State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China |
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| Abstract: | Twenty-one compounds were isolated from an ethanol extract of Machilus wangchiana by a combination of various chromatographic techniques including column chromatography over silica gel and Sephadex LH-20 and reversed-phase HPLC.Their structures were identified by spectroscopic data analysis including optical rotation,UV,IR,MS,and NMR data.The compounds are categorized as eight butanolides(1-8),eight lignans(9-16),and five terpenoids(17-21).Compound 16 is a new natural product with an uncommon heptanorlignan skeleton.Meanwhile,the unique Ginkgo biloba(maidenhair) metabolites ginkgolides A(19) and ginkgolides B(20) were obtained from this material.In the preliminary assays,compound 5 showed selective inhibitory activities against human stomach cancer cells(BGC-823) and ovary cancer cells(A2780) with IC50 values of 0.13×10-6 and 2.66×10-6 mol·L-1,respectively.Compounds 8 and 9,at 1×10-5 mol·L-1,showed inhibitory activities against the release of β-glucuronidase of the polymorphous nuclear leukocytes induced by platelet activating factor(PAF),with inhibition rates of 60.0% and 54.2%. |
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| Keywords: | Machilus wangchiana butanolide lignans terpenoids cytotoxicity inhibitory activity of β-glucuronidase release |
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