黄连解毒汤对2型糖尿病大鼠血管内皮功能的影响

目的:通过观察黄连解毒汤对2型糖尿病大鼠血管内皮功能的影响,探讨黄连解毒汤对2型糖尿病血管并发症的防治意义及其机制。方法:采用小剂量链脲佐菌尾静脉注射加高糖高热量饲料喂养的方法建立2型糖尿病大鼠模型,将模型动物随机分为模型组、黄连解毒汤组、阿司匹林组,另设正常对照组。药物干预治疗9周后,各组动物进行口服糖耐量实验(OGTT);10周后,观察各组动物体重变化并检测各组空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)、空腹血清胰岛素(FINS)值及血清一氧化氮(NO)、血浆内皮素(ET)、血管紧张素Ⅱ(AngⅡ),von Willebrand因子(vWF)的表达水平。结果:黄连解毒汤组OGTT较模型组改善,体重,TC,TG,ET值均低于模型组(P<0.05),HDL-C和NO值高于模型组(P<0.05);FBG,FINS,AngⅡ,vWF值均显著低于模型组(P<0.01)。与阿司匹林组相比,黄连解毒汤组FBG值显著降低(P<0.05),TG,HDL-C,NO,AngⅡ,vWF的改善情况优于阿司匹林组,但无显著性差异(P>0.05)。结论:黄连解毒汤对2型糖尿病早期出现的血管内皮损伤有良好的保护作用,机制与其降糖、降脂、抗炎、改善胰岛素抵抗及内皮依赖性的舒张血管等作用有关。

Protective effects of Huanglian Jiedu decoction on vascular endothelial function in type 2 diabetic rats

OBJECTIVE: To investigate the effects of Huanglian Jiedu (HLJD) decoction on vascular endothelial function in type 2 diabetic rats and explore the prophylactic and therapeutic significance and pharmacological mechanism of HLJD decoction in type 2 diabetic angiopathic complication. METHOD: The murine type 2 diabetes models were induced by the intravenous injection of a small dose of streptozotocin plus high fat and high caloric laboratory chow. Then modeled diabetic animals were divided into model group, HLJD group, and aspirin group. Normal ratsfed with routine chow were designated as normal group. The oral glucose tolerance test (OGTT) were performed in all animals, 9 weeks after treatment, the changes of murine body weights and levels of fasting blood glucose (FBG), serum total cholesterol (TC), triglycerides (TG), high density lipoprotein-cholesterol(HDL-C), fasting serum insulin (FINS), serum nitric oxide, plasma endothelin, angiotensin II and von Willebrand Factor (vWF) were determined 10 weeks after treatment. RESULT: Compared with model group, the result of OGTT of HLJD group was improved. The levels of the body weights, TC, TG and ET in HLJD group weredecreased (P < 0.05). The levels of FBG,INS, AngII and vWFwere significantly decreased (P < 0.01), and the levels of HDL-C and NO were obviously increased (P < 0.05), as compared with those in model group. Furthermore. The levels of FBG was lower in HLJD group than in aspirin group (P < 0.05), and the improvement of TG, HDL-C,NO, AngII, vWF levels in HLJD group was more greatly than that in aspirin group, but there was not significant difference between two groups (P > 0.05). CONCLUSION: It is suggested by the present results that HLJD decoction could protect vascular endothelium from early damage in type 2 diabetes. The protective effects of HLJD on endothelium might be related to its ability of reducing the blood glucose, adjusting plasma lipids profiles, improving insulin resistance, antagonizing inflammatory mediators and inducing endothelium-dependent vascular relaxation.