HPLC测定附子及其炮制品中3种双酯型生物碱的含量

目的：建立同时测定附子中3种双酯型生物碱——新乌头碱、乌头碱、次乌头碱含量的HPLC分析方法。方法：Zorbax Eclipse XDB ₁₈柱,40 mmol乙酸铵缓冲液-乙腈,梯度洗脱,流速1.0 mL·min^-1,检测波长230 nm。结果：3种双酯型生物碱新乌头碱、乌头碱、次乌头碱均达到了较好的分离,分别在0.035 7～1.784 μg（r=0.999 9）,0.012 6～0.632 μg（r=0.999 7）和0.033 4～1.672 μg（r=0.999 7）呈良好的线形关系。结论：该方法简便、快速、灵敏,可有效地用于附子的鉴别和内在质量控制。

Determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products by HPLC

OBJECTIVE: To establish an HPLC method for the determination of three kind of diester diterpenoid alkaloids (DDAs) in Aconitum carmichaeli and its processed products namely mesaconitine, aconitine and hypaconitine. METHOD: A Zorbax Eclipse XDB C18 column was used, and ammonium acetate solution-acetonitrile as the mobile phase, with a flow rate of 1.0 mL x min(-1) and a detection wavelength of 230 nm. RESULT: Mesaconitine, aconitine and hypaconitine, were separated, and the calibration curves of them were in good linearity over the range of 0.035 7-1.784 microg (r = 0.9999), 0.0126-0.632 microg (r = 0.9997) and 0.0334-1.672 microg (r = 0.9997) respectively. CONCLUSION: This method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.