塞隆骨原动物高原鼢鼠核基因18SrRNA序列测定与分析

目的 :测定仓鼠科动物高原鼢鼠Myospalax baileyi的核rDNA基因序列 ,为塞隆骨正品基原检定提供分子依据。方法 :采用PCR直接测序技术测定高原鼢鼠 18S rRNA基因核苷酸序列并作序列特征分析。结果 :高原鼢鼠的 18Sr RNA序列长度为 1851bp。根据排序比较 ,高原鼢鼠与 2种鼠科动物间的DNA序列同源性为 72.04%～72.18%。结论 :通过基因序列分析 ,DNA测序技术可成为塞隆骨正品基原检定的准确有效手段。

Nuclear Ribosomal RNA Small Subunit (18S rRNA) Nucleotide Sequencing and Characterization of Sailonggu(Whole Bone of Myospalax baileyi Thomas)

OBJECTIVE: Sequencing the nuclear ribosomal RNA small subunit (18S rRNA) gene of Myospalax baileyi (Cricetidae) to develop an ultimate and definitive means for origin identification of genuine Sailonggu. METHODS: The total DNA was prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences was generated using software program Genetyx-SV/R Version 10.1. RESULTS: The entire 18S rRNA gene region of M. baileyi spanded 1851 bp in length. Although multiple alignment of sequence indicates that there are only lower homology (72.04%-72.18%) comparing with its two alias Mus musculus (GenBank Accession number X00686) and Rattus norvegicus (M11188) (Muridae), their highly conservative domain is located in 1020-1509 nt. There are many variable sites from upstream of 5'-end, which could provide a novel information for molecular recognition of Sailonggu. CONCLUSION: DNA sequencing could be a useful and reliable tool in the origin identification of genuine Sailonggu.