| 氧化苦参碱对人脐静脉平滑肌细胞钙化的影响及其可能机制 |
| |
| 引用本文： | 王秀梅,张娟,张鸣号,刘爽,李桂忠,曹军.氧化苦参碱对人脐静脉平滑肌细胞钙化的影响及其可能机制[J].中国中药杂志,2012,37(7):1002-1006. |
| |
| 作者姓名： | 王秀梅 张娟 张鸣号 刘爽 李桂忠 曹军 |
| |
| 作者单位： | 宁夏医科大学 基础学院 病理生理学教研室,宁夏 银川,750004 |
| |
| 基金项目： | 宁夏教育厅项目(宁教高[2007]292号);宁夏医科大学重点项目(XZ2009004) |
| |
| 摘 要： | 目的:观察氧化苦参碱(oxymatrine,OMT)对人脐静脉平滑肌细胞(humans umbilical vein smooth muscle cells,HUSMCs)钙化的影响及其可能机制。方法:以β-甘油磷酸盐诱导HUSMCs钙化,实验分为对照组、钙化组、单纯OMT组、OMT干预高、中、低剂量组。采用Von Kossa染色鉴定细胞钙化,比色法检测细胞钙含量,磷酸苯二钠法测定ALP活性,放射免疫法测定骨钙素(OC)含量,ELISA检测细胞培养液中TGF-β1和细胞内psmad2/3,smad2/3的含量变化,Western blot法检测细胞内Cbfα1蛋白的表达。结果:钙化组与对照组相比平滑肌细胞内可见大量黑色颗粒聚集,钙含量,ALP活性,OC,TGF-β1,smad2/3磷酸化和Cbfα1蛋白的含量均明显增加;OMT干预后钙化指标均下降,TGF-β1,smad2/3磷酸化和Cbfα1蛋白表达量亦明显减少,且高剂量OMT组效应强于中、低剂量组。结论:OMT可有效抑制β-甘油磷酸盐诱导的HUSMCs钙化,且OMT降低TGF-β1,smad2/3磷酸化和Cbfα1蛋白表达可能是OMT抑制HUSMCs钙化的机制之一。
|
| 关 键 词： | 血管钙化 氧化苦参碱 人脐静脉血管平滑肌细胞 |
| 收稿时间： | 6/9/2011 12:00:00 AM |
Effect of oxymatrine on vascular calcification of humans umbilical vein smooth muscle cells and its underlying mechanism |
| |
| WANG Xiumei,ZHANG Juan,ZHANG Minghao,LIU Shuang,LI Guizhong and CAO Jun.Effect of oxymatrine on vascular calcification of humans umbilical vein smooth muscle cells and its underlying mechanism[J].China Journal of Chinese Materia Medica,2012,37(7):1002-1006. |
| |
| Authors: | WANG Xiumei ZHANG Juan ZHANG Minghao LIU Shuang LI Guizhong and CAO Jun |
| |
| Institution: | Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China;Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China;Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China;Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China;Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China;Department of Pathophysiology, Ningxia Medical University, Yinchuan 750004, China |
| |
| Abstract: | Objective: To observe the effect of oxymatrine(OMT) on calcification of humans umbilical vein smooth muscle cells and its underlying mechanism.Method: Human umbilical vein smooth muscle cells(HUSMCs) were calcified by β-giycerophosphosphate(β-GP) and then divided into 6 groups: the control group,the calcification group,the pure OMT group,and lower,middle and higher-dosage OMT groups.Cell calcification were observed by Von Kossa staining,calcium content in HUSMCs were determined by the colorimetric method,the alkaline phosphatase(ALP) activity in HUSMCs were determined by phenyl diphosphate-2-sodium,the osteocalcin(OC) level in HUSMCs were determined by radioimmunossay,the transforming growth factor-β1(TGF-β1) level in HUSMC culture medium and the content changes in psmad2/3 and smad2/3 were determined by the ELISA method,and the expression of Core binding factor α 1(Cbfα1) protein in HUSMCs were determined by western blot method.Result: Compared with the control group,the calcification group showed a great number of black granules among the smooth muscle cells and significant increase in the content of calcium and OC and the activity of ALP;OMT intervention can decrease the content of calcium,OC,TGF-β1,psmad2/3 and Cbfα1 and the activity of ALP.And high-dosage OMT group had better effect than middle and low-dosage groups.Conclusion: OMT can effectively inhibit β-GP-induced HUSMC calcification and its effect on reducing TGF-β1,psmad2/3 and Cbfα1 may be one of its mechanisms in inhibiting HVSMC calcification. |
| |
| Keywords: | vascular calcification oxymatrine humans umbilical vein smooth muscle cells |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
