梓葛冻干粉针对气虚血瘀证大鼠凝血功能、血液流变学和NO的影响

目的:探讨复方中药新药制剂梓葛冻干粉针对气虚血瘀证大鼠凝血功能、血液流变学及NO的改善作用。方法:采用饥饿、疲劳、寒湿、惊恐和高脂饮食相结合的方法复制气虚血瘀证模型,玻片法测凝血时间(CT),断尾法测出血时间(BT),同时评价梓葛冻干粉针对凝血酶原时间(PT)、活化部分凝血活酶(APTT)和凝血酶时间(TT)的影响;电阻法测红细胞压积,全自动血液流变仪测流变学指标;酶法测定血清中NO。结果:梓葛冻干粉针65.40 mg·kg-1能显著延长大鼠CT,BT,PT,APTT,TT;能显著降低血液流变学各项指标;提高血清中NO的水平。32.70 mg·kg-1能明显延长大鼠CT,PT,APTT;降低全血黏度、红细胞聚集指数和全血还原黏度。结论:梓葛冻干粉针具有改善凝血功能、血液流变学指标以及升高血清中NO含量的作用。

Effect of catalpol and puerarin freeze-dried powder on coagulability, hemorheology and No in rats with Qi-deficiency and blood-stasis syndrome

Objective: To study the effect of catalpol and puerarin freeze-dried powder for injection (CPFPI), a new compound traditional Chinese medicine(TCM) preparation, on coagulability, hemorheology and NO in rats with qi-deficiency and blood-stasis syndrome. Method: The model of rats with qi-deficiency and blood-stasis syndrome was established by hunger, fatigue, cold-dampness, panic and high fat diet. Coagulation time (CT) was observed by the glass method, and bleeding time (BT) was measured by tail-cutting method. The effects of CPFPI were also evaluated with prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). HCT was measured by the electric resistance method, hemorheology indicators were observed by auto-hemorheological instrument. The level of NO in blood serum was measured by NO assay kit. Result: CPFPI 65.40 mg·kg^-1 significantly prolonged CT, BT, PT, APTT and TT in rats. The viscosity of whole blood and plasma, hematocrit, erythrocyte aggregation and rigidity index, and reduced viscosity of whole blood in 65.40 mg·kg^-1 groups were lower than model group. CPFPI 65.40 mg·kg^-1 can raise the level of NO in blood serum.32.70 mg·kg^-1 markedly prolonged CT, PT and APTT and decreased whole blood viscosity, erythrocyte aggregation index and whole blood reduction viscosity. Conclusion: CPFPI has a significant effect in improving coagulability and hemorheology index and enhancing NO content in blood serum.