天花粉药材及其类似品的分子鉴别新方法

目的：寻找天花粉类药材快捷准确的分子鉴别方法。方法：在天花粉ITS保守区域设计3个20 mer左右单引物,对19批天花粉商品及其9种伪品进行PCR扩增。结果：引物TkS1-64得到天花粉及其伪品的DNA多态性图谱,其中560 bp和960 bp条带为天花粉的特征鉴别条带。结论：引物TkS1-64具有稳定性好、重复性高的优点,可以作为天花粉类药材分子鉴别引物。此方法定名为锚定引物扩增多态性DNA（anchored primer amplification polymorphism DNA,简称APAPD）,在中药材鉴别中具有推广应用的价值。

A new molecular method to authenticate Radix Trichosanthis as well as its adulterants and substitutes

Objective:To explore a new molecular method to authenticate Radix Trichosanthis.Method:Three 20 mer prim- ers based on the ITS sequence was designed.The PCR reaction system was optimized and applied to nineteen different sources of Radix Trichosanthis and nine adulterants and substitutes.Result:Polymorphic map of Radix Trichosanthis and its adulterants was obtained from primer TKS1-64.560 bp and 960 bp bands were authentic markers for Radix Trichosanthis.Conclusion:Primer TKSI-64F pos- sesses the advantages of good stability and reproducibility.This new method is named as anchored primer amplification polymorphism DNA(APAPD).It was a potential method to used in molecular identification of other meteria medica.