丹参对高胆固醇血症患者内皮祖细胞数量及功能的影响

目的：观察丹参对高胆固醇血症患者内皮祖细胞(endothelial progenitor cells,EPCs)数量及功能的影响。方法：24例高胆固醇血症患者随机分为2组,对照组常规治疗,治疗组除常规治疗外另接受2周复方丹参滴丸治疗,结束后各取空腹外周血20 mL贴壁选择法行EPCs培养,采用流式细胞术、倒置显微镜、MTT法、Boyden小室等分别观察其对EPCs数量、集落形成能力、增殖能力、黏附能力及迁移能力的影响。结果：复方丹参滴丸对高胆固醇血症患者外周血EPCs数量无显著性影响,但显著提高其克隆形成能力、增殖能力、黏附能力及迁移能力。结论：复方丹参滴丸显著改善高胆固醇血症患者内皮祖细胞功能。

Effects of Danshen on number and activity of endothelial progenitor cells of patients with Hypercholesterolemia

Objective: To investigate the effects of Danshen on number and activity of endothelial progenitor cells(EPCs)of patients with Hypercholesterolemia. Method: 24 patients with Hypercholesterolemia were randomLy divided into 2 groups: control group (n=12), and treatment group (n=12, receiving Composite Denshen Pilulae, 10# tid for 2 weeks). after 2 weeks, 20 mL peripheral blood was obtained from each patient, Mononuclear fraction of human peripheral blood was obtained by density gradient centrifugation, plated on fibronectin coated culture dishes. The cells were identified by immunohistochemistry and flow cytometry and tested the ability to intake ac-LDL. Cell clusters were viewed with an inverted microscope, fluorescence-activated cell sorting (FACS) analysis of PE-CD34 and FITC-AC133 was performed to detect number of EPCs, EPC proliferation and migration were assayed with MTT assay, modified Boyden chamber assay. EPCs adhesion ability assay was performed by replating cells on fibronectin-coated dishes, and then counting adherent cells. Result: Numbers of EPCs (10³ cells per 1 mL peripheral blood) of treatment group was higher than control group (7.20±1.29 vs 6.88±1.00). Compared with group control, numbers of clusters (per 40 power microscopic field), adhesive EPCs (per 400 power microscopic field) and migratory EPCs (per 200 power microscopic field) of treatment group were significantly increased (4.47±0.94 vs 3.38±0.57, P<0.01, 11.81±2.29 vs 10.03±1.32, P<0.05 and 15.75±2.27 vs 11.95±1.28, P<0.01, respectively), while OD vallue of treatment group were significantly increased too(0.27±0.04 vs 0.20±0.03, P<0.01). Conclution: Danshen can significantly enhance EPCs functional activity of patients with Hypercholesterolemia.