莪术不同种和居群的ISSR-PCR分析

目的：应用ISSR-PCR标记技术研究不同种及居群莪术的亲缘关系和遗传多样性。方法：对蓬莪术、广西莪术和温郁金共8个居群的37个样本进行ISSR-PCR分析，用POPGEN32软件分析其遗传相似系数及遗传距离，UPGMA方法聚类，构建亲缘关系系统图。结果：5条引物共扩增出65个位点，其中34个位点是多态性位点，多态位点百分率为52.3％。遗传相似系数变化范围0.686 4～0.999 7。Nei's基因多样性(H)指数为0.152 1，Shannon信息指数(I)为0.233 8，莪术种内的遗传多样性低于种间。结论：莪术居群间的遗传变异较大，而居群内部的分化程度很低。不同居群的莪术与种的特性和地理分布有一定相关性，为莪术的品种鉴定及栽培育种提供了分子生物学依据。

ISSR-PCR analysis in different species and populations ofRhizoma Curcumae

Objective: To study the genetic diversity and genetic relationship in different species and populations of Curcuma by ISSR-PCR marker technique. Method: Eighty populations and 37 samples of Curcuma including C. phaeocaulis, C. kwangsiensis and C. wenyujin were studied by ISSR-PCR markers. The systematic diagram of Similar coefficient and genetic distance were set up by POPGEN32 software and clustered by UPGMA method. Result: A total of 65 loci were scored by 5 primers, among which 34 were polymorphic loci. The percentage of polymorphic loci was 52.3%. Genetic similarity coefficient changed from 0.686 4 to 0.999 7. Nei's gene diversity index(H), and Shannon information index(I) were 0.152 1 and 0.233 8. The inner genetic diversity of Curcuma species was lower than the outer. Conclusion: The genetic variation of different populations Curcuma was big. The inherited differentiation of inner populations was low. Different populations of Curcuma were related to character of species and geological distribution.