| 小花异裂菊中的芳香类化学成分及其活性 |
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| 引用本文： | 樊晓娜,林生,朱承根,刘洋,胡金凤,陈晓光,王文杰,陈乃宏,石建功.小花异裂菊中的芳香类化学成分及其活性[J].中国中药杂志,2011,36(1):48-56. |
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| 作者姓名： | 樊晓娜 林生 朱承根 刘洋 胡金凤 陈晓光 王文杰 陈乃宏 石建功 |
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| 作者单位： | 中国医学科学院北京协和医学院药物研究所中草药物质基础与资源利用教育部重点实验室,北京,100050 |
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| 基金项目： | 国家自然科学基金项目(30825044,20932007);国家科技重大专项(2009ZX09301-003-4-1) |
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| 摘 要： | 目的:研究异裂菊属Heteroplexis植物小花异裂菊的化学成分.方法:采用乙醇提取、乙酸乙酯萃取、硅胶柱色谱、大孔树脂柱色谱、Pharmadex LH-20柱色谱、反相中压柱色谱、闪式柱色谱以及反相HPLC柱色谱分离;运用波谱数据测定和分析鉴定化合物的结构;采用人肿瘤细胞、HIV-1复制、MPP+诱导PC12细胞损伤和多形核白细胞β葡萄糖苷酸酶释放药理模型筛选其活性.结果:分离鉴定了31个化合物,包括12个苯丙素及其衍生物,(+)-(7S,8R)-3-甲氧基-4-羟基苯基丙三醇(1),阿魏酸(2),肉桂酸甲酯(3),1-二十醇-3,4-二羟基肉桂酸酯(4),morinin B(5),sinapyl diangelate(6),绿原酸(7),4-O-咖啡酰奎尼酸(8),5-O-咖啡酰奎尼酸(9),5-O-咖啡酰奎尼酸甲酯(10),1,5-O-二咖啡酰奎尼酸(11)和4,5-O-二咖啡酰奎尼酸甲酯(12);3个木脂素类,(+)-松脂素(13),青刺尖木脂醇(14)和(+)-松脂素-O-β-D-吡哺葡萄糖苷(15);4个苯乙酮类,2,4-二乙酰基茴香醚(16),espeleton(17),viscidone(18),12-羟基佩兰毒素-12-O-β-D-吡喃葡萄糖苷(19);9个黄酮类,异樱花素(20),橙皮素(21),3-甲氧基-5,7,3',4'-四羟基黄酮(22),金合欢素(23),5-羟基-7,4'-二甲氧基黄酮(24),7-甲氧基-4',5,6-三羟基黄酮(25),3,3'-二甲基槲皮素(26),山柰酚3-O-芦丁糖苷(27),芦丁(28);3个香豆素类,东莨菪内酯(29),7-羟基香豆素(30)和泽兰内酯(31).化合物6,22分别对人胃癌细胞(BGC-823)和人肺腺癌细胞(A549)具有一定的选择性生长抑制作用,IC50值分别为3.74×10-5,7.17×10-5mol·L-1;化合物6有抑制HIV-1病毒复制的活性,IC50值为4.04×10-6 mol·L-1;在1.0×10-5mol·L-1浓度下,化合物22对MPP+致PC12细胞损伤有保护作用,与模型组比较有显著性差异(P<0.01);化合物26和31能抑制PAF刺激的多形核白细胞β葡萄糖苷酸酶释放,抑制率分别为75.6%(P<0.001)和53.9%(P<0.01).结论:化合物1-31均为首次从异裂菊属植物中分离得到;化合物6和22分别对人胃癌细胞(BGC-823)和人肺腺癌细胞(A549)具有选择性生长抑制作用,化合物6有抑制HIV-1病毒复制的活性,化合物22具有神经细胞保护活性,化合物26和31有潜在抗炎活性,其他化合物在测试浓度下在以上筛选模型中未显示明显活性.
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| 关 键 词： | 异裂菊属 小花异裂菊 化学成分 活性筛选 |
| 收稿时间： | 2010/7/26 0:00:00 |
Aromatic constituents of Heteroplexis micocephal and their bioactivities |
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| FAN Xiaon,LIN Sheng,ZHU Chenggen,LIU Yang,HU Jinfeng,CHEN Xiaoguang,WANG Wenjie,CHEN Naihong and SHI Jiangong.Aromatic constituents of Heteroplexis micocephal and their bioactivities[J].China Journal of Chinese Materia Medica,2011,36(1):48-56. |
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| Authors: | FAN Xiaon LIN Sheng ZHU Chenggen LIU Yang HU Jinfeng CHEN Xiaoguang WANG Wenjie CHEN Naihong and SHI Jiangong |
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| Institution: | Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China |
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| Abstract: | Objective: To investigate the chemical constituents of Heteroplexis microcephala and their bioactivities.Method: The constituents were isolated by using a combination of various chromatographic techniques including column chromatography over macroporous adsorbent resin,silica gel,Pharmadex LH-20,and C-18,as well as reversed-phase HPLC.Structures of the isolates were identified by spectroscopic data analysis.In vitro cytotoxic,HIV-1 replication,neuroprotective,and anti-inflammatory activities were screened b... |
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| Keywords: | Heteroplexis Heteroplexis micocephal chemical constituents bioactivities |
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