星点设计-效应面法优选荷叶中荷叶碱与 荷叶黄酮的提取工艺

目的：优化荷叶中2类有效成分的醇提工艺。方法：采用星点设计，以荷叶碱，荷叶总黄酮的提取率为指标，对2种指标进行归一化处理，采用效应面法优化荷叶的提取工艺条件。结果：确定最优提取工艺为75%～80%乙醇，提取时间120～180 min， 加醇量 20～25倍，提取2次，荷叶碱和荷叶总黄酮提取率的实际值与预测值的偏差分别为5.53%，-6.02%。结论：采用星点设计-效应面法优选荷叶的醇提工艺，实际值与预测值吻合度高，预测性良好，是可行的。

Optimal extraction of nuciferine and flavone from lotus leaf based on central composite design and response surface methodology

Objective: To optimize the process of extracting effective constituents from lotus leaf. Method: Independent variables were ethanol concentration reflux time and solvent fold, dependent variables were extraction rates of nuciferine and flavone in lotus leaf, central composite design and response surface methodology were used for optimization of extraction of lotus leaf. Result: The optimum conditions of extraction process were 75%-80% ethanol, 2-3 hours for reflux, 20-25 fold solvent and 2 times for extraction. Bias between observed and predicted of rates of nuciferine and flavone values were 5.53%, -6.02%, respectively. Conclusion: The values observed and predicted were close to each other, which proved that the optimization of of extraction of lotus leaf by central composite design and response surface methodology was reasonable and successful.