首页 | 本学科首页   官方微博 | 高级检索  
检索        

淫羊藿苷对HCY诱导增殖血管平滑肌细胞GRP78表达的影响
引用本文:沈晓君,何航.淫羊藿苷对HCY诱导增殖血管平滑肌细胞GRP78表达的影响[J].中国中药杂志,2009,34(15):1964-1967.
作者姓名:沈晓君  何航
作者单位:河南中医学院,基础医学院,河南,郑州,450008
基金项目:河南省科技攻关计划项目(0624420026)
摘    要:目的:研究淫羊藿苷(ICA)对同型半胱氨酸(HCY)诱导的增殖血管平滑肌细胞(VSMC)葡萄糖调节蛋白78(GRP78)基因表达的影响,探讨ICA抗动脉粥样硬化的机制.方法:建立HCY诱导的兔VSMC增殖模型,与不同浓度的ICA共同培养48 h后,用流式细胞仪Annexin/PI双染法检测凋亡率;RT-PCR检测GRP78 mRNA的表达;目的基因克隆、鉴定并测序.结果:0.2,0.4 mg·L~(-1)的IcA可显著促进兔VSMC凋亡,且存在剂量依赖性,与半胱氨酸组相比,差异显著(P<0.01);RT-PCR结果显示:0.2,0.4 mg·L~(-1)的淫羊藿苷作用于VSMC 48 h后,GRP78 mRNA表达水平显著升高,与半胱氨酸组相比差异显著(P<0.05);测序结果表明,来源于VSMC的全长648 bp的基因片断与兔葡萄糖调节蛋白78基因高度同源.结论:ICA促进GRP78 mRNA表达,诱导兔VSMC凋亡,可能是其抗动脉粥样硬化的机制之一.

关 键 词:淫羊藿苷  同型半胱氨酸  血管平滑肌细胞  葡萄糖调节蛋白78  细胞凋亡
收稿时间:2009/2/10 0:00:00

Effects of icariin on expression of glucose regulated protein78 in vascular smooth muscle cell induced by homocysteinemia
SHEN Xiaojun and HE Hang.Effects of icariin on expression of glucose regulated protein78 in vascular smooth muscle cell induced by homocysteinemia[J].China Journal of Chinese Materia Medica,2009,34(15):1964-1967.
Authors:SHEN Xiaojun and HE Hang
Abstract:Objective: To investigate the effects of icariin on the GRP78 mRNA expression of vascular smooth muscle cell in vitro and to elucidate the anti-atherosclerosis mechanism of icariin. Method: The model was established by using halfcystine in vitro. The vascular smooth muscle cell had been incubated with various concentrations of icariin for 48 hours. The apoptosis rate was detected by flow cytometry using Annexin/PI-staining and GRP78 mRNA expression was tested by RT- PCR. These differentially expressed fragments were cloned, identified and sequenced. Result: Icariin at concentrations of 0.2 and 0.4 mg·L-1 obviously promoted the apoptosis of vascular smooth muscle cell. The early apoptosis rate was raised magnificently in a dose dependent manner compared with that of the halfcystine group (P<0.01). Furthermore, the fragment, 648 bp, was similar to the gene of glucose regulated protein GRP78 from VSMC. Icariin could magnificently increase the GRP78 mRNA expression compared with that of the halfcystine group (P<0.05). Conclusion: The anti-atherosclerosis mechanism of icariin might be partly due to the promotion of apoptosis and the promotion of the GRP78 mRNA expression of vascular smooth muscle cell.
Keywords:icariin  homocysteinemia  vascular smooth muscle cell  glucose regulated protein78  cell apoptosis
本文献已被 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号