柳茶多糖对小鼠免疫功能的影响

目的:研究柳茶多糖对免疫低下小鼠免疫功能的影响.方法:取正常昆明种小鼠200只,雌雄各半,随机分为5组:空白对照组、模型组、柳茶多糖低剂量组(110 mg·kg-1)、中剂量组(220 mg·kg-1)和高剂量组(440 mg·kg-1).腹腔注射环磷酰胺制备免疫低下的小鼠模型,测定柳茶多糖对小鼠免疫器官质量、小鼠迟发型变态反应(DTH)的影响;血清溶血素法测定体液免疫功能;碳粒廓清法测定非特异性免疫功能;脾淋巴细胞增殖试验测定细胞免疫功能.结果:110～440 mg·kg-1的柳茶多糖能显著提高免疫器官质量,提高DTH和促进其血清溶血素lgG,lgM的含量,增强小鼠的碳粒廓清率,明显改善免疫低下小鼠的脾淋巴细胞增殖反应.结论:柳茶多糖能增加免疫低下小鼠的免疫器官质量,对非特异性免疫、迟发型变态反应、体液免疫以及细胞免疫功能均有明显的促进作用.

Effect of polysaccharides in processed Sibiraea on immunologic function of immunosuppression mice

Objective: To study the effect of polysaccharides in processed Sibiraea on the immunologic function of immunosuppression mice. Method: The immunosuppressed mice were induced by cyclophosphamide. After the treatment, the organ weight index and the delayed type hypersensitivity of the mice were investigated. The humoral immune function was determined by serum hemolysin assay. Non-specific immune function was determined by carbon clearance method. Cellular immune function was determined by spleen lymphocyte proliferation test. Two hundred kunming mice were randomly divided into five groups: normal controls, model group, low-dose group(110 mg·kg^-1), middle-dose group(220 mg·kg^-1),high-dose group(440 mg·kg^-1). Drugs were given to the mice by oral gavage every day. Result: The immunosuppressed mice treated with Sibiraea polysaccharide at intragastrica dose of 110-440 mg·kg^-1 have increased weight of the immune organs, increased content of DTH and content in serum hemolysin lgG and lgM. Mean while the rate of carbon clearance was enhanced and the proliferation of spleen lymphocyte was increased. Conclusion: Polysaccharides in processed Sibiraea can increase the weight of the immune organs. At the same time, non-specific immune, DTH, humoral immune and cellular immune function were enhanced significantly.