| 用于评价致敏原的IgG-HepG2细胞激活过程中相关炎性因子表达的观察 |
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| 引用本文： | 卢希,王明玮,雷帆,王玉刚,袁梽漪,赵爽,李慧玉,邢东明,杜力军.用于评价致敏原的IgG-HepG2细胞激活过程中相关炎性因子表达的观察[J].中国中药杂志,2011,36(14):1855-1859. |
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| 作者姓名： | 卢希 王明玮 雷帆 王玉刚 袁梽漪 赵爽 李慧玉 邢东明 杜力军 |
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| 作者单位： | 1. 清华大学 医学院 生命科学学院 教育部蛋白质重点实验室,北京,100084
2. 新疆军区西安第二干休所 医务室,陕西西安,710100 |
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| 基金项目： | 国家自然科学基金项目(30801523,30973896,81073092);国家"重大新药创制"科技重大专项(2009ZX09308-003,2009ZX09502-021,2009ZX09502,2011ZX09101-002-11) |
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| 摘 要： | 目的 研究转IgG启动子调控GFP基因表达HepG2细胞激活过程中相关炎性因子表达的变化.方法 采用Elisa法评价葛根素和LPS后诱导IgG启动子转基因细胞分泌IL-1β,IL-8,TNF-α和MCP-1蛋白量,qPCR法评价炎性相关因子和固有免疫相关因子的mRNA转录表达量.结果 和HepG2细胞相比,IgG启动子转基因细胞不增加炎性因子分泌和基因表达量,不激活固有免疫.葛根素不增加转基因细胞炎性因子分泌和基因表达量,不激活固有免疫系统.LPS激活固有免疫系统,显著升高IL-8,TNF-α和MCP-1分泌量.结论 IgG启动子转基因HepG2细胞可以作为评价Ⅱ型变态反应的特异性细胞模型,提示葛根素可以作为特异性激活IgG启动子的阳性对照品.
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| 关 键 词： | IgG启动子 致敏原 炎性因子 |
| 收稿时间： | 2011/6/12 0:00:00 |
Investigation of inflammasome during excitation of IgG-HepG2 cells for evaluation of allergenic ingredients |
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| LU Xi,WANG Mingwei,LEI Fan,WANG Yugang,YUAN Zhiyi,ZHAO Shuang,LI Huiyu,XING Dongming and DU Lijun.Investigation of inflammasome during excitation of IgG-HepG2 cells for evaluation of allergenic ingredients[J].China Journal of Chinese Materia Medica,2011,36(14):1855-1859. |
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| Authors: | LU Xi WANG Mingwei LEI Fan WANG Yugang YUAN Zhiyi ZHAO Shuang LI Huiyu XING Dongming and DU Lijun |
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| Institution: | Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Medical Service, Xi'an the Second Sanatorium, Xinjiang Province, Xi'an 710100, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Protein Science Laboratory of the Ministry of Education, Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China |
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| Abstract: | |
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| Keywords: | IgG promoter allergenic ingredients inflammasome |
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