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道地药材江枳壳品种遗传学关系的ISSR证据
引用本文:左云娟,,朱培林,刘强,陈春华,周世良.道地药材江枳壳品种遗传学关系的ISSR证据[J].中国中药杂志,2005,30(18):1416-1419.
作者姓名:左云娟    朱培林  刘强  陈春华  周世良
作者单位:1. 中国科学院,植物研究所,系统与进化植物学重点实验室,北京,100093;云南大学生命科学学院,云南,昆明,650091;中国科学院,研究生院,北京,100039
2. 汇仁集团有限公司,江西,南昌,330052;江西省宜春市中药材技术中心,江西,宜春,336000
3. 汇仁集团有限公司,江西,南昌,330052
4. 中国科学院,植物研究所,系统与进化植物学重点实验室,北京,100093
摘    要:1.中国科学院植物研究所系统与进化植物学重点实验室 北京100093; 2.汇仁集团有限公司 江西南昌330052; 3.江西省宜春市中药材技术中心 江西宜春336000; 4.云南大学生命科学学院 云南昆明650091; 5.中国科学院植物研究所系统与进化植物学重点实验室 北京100039

关 键 词:江枳壳  ISSR  品种  遗传关系
文章编号:1001-5302(2005)18-1416-04
收稿时间:2004-10-19
修稿时间:2004-10-19

Genetic relationships among Fructus Aurantii cultivars revealed by ISSR
ZUO Yun-juan;ZHU Pei-lin;LIU Qiang;CHEN Chun-hua;ZHOU Shi-liang.Genetic relationships among Fructus Aurantii cultivars revealed by ISSR[J].China Journal of Chinese Materia Medica,2005,30(18):1416-1419.
Authors:ZUO Yun-juan;ZHU Pei-lin;LIU Qiang;CHEN Chun-hua;ZHOU Shi-liang
Institution:1. laboratory of Systematic and Evolutionary Botany, Institute of Botany, The Chinese Academy of Sciences, Beifing 100093, China ; 2. Huiren Croup Co., It, d, Nanchang 330052, China; 3. Traditional Chinese Medicine Service Center, Yichun 336000, China; 4. School of Life Sciences, Yunnan Uiniversity, Kunming 650091, China; 5. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Abstract:Objective: A total of 27 samples belonging to 5 cultivars of Fructus Aurantii (Citrus aurantium), i. e. cv. Xiucheng, cv. Xiangcheng, cv. Lecheng, cv. Jizicheng, and cv. Youzicheng, collected at Changfu and Huanggang, Zhangshu City, Jiangxi Province, were assayed to reveal the genetic relationship among the cultivars and the accordance between morphological and molecular markers. Method: Cultivar identification was based on morphology and cultivar relationship was based on Inter-simple sequence repeats (ISSR). Result:Twenty out of 40 ISSR primers screened generated 392 loci across all 27 samples with 315 informative loci. The UPGMA dendrogram showed that samples within cv. Xiucheng and cv. Xiangcheng from Changfu were closely related. However, samples of cv. Lecheng, cv. Jizicheng and cv. Youzicheng from Huanggang, or cv. Xiucheng and cv. Xiangcheng from both Changfu and Huanggang did not exhibited close relationships within each cultivars. Conclusion:Based on morphology the same cultivar grown in different plantations, or even within a single plantation sometimes do not show close genetic relationship, indicating diverse origin of the cultivars. Synonyms or homonyms are believed to common phenomenon in Fructus Aurantii production. To solve the problem ISSR markers can serve a kind of molecular markers which are preferable to partition genetic variations within and between cultivars and to establish genetic relationships among them.
Keywords:Fructus Aurantii  Citrus aurantium  cultivar  ISSR  genetic relationship
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