首页 | 本学科首页   官方微博 | 高级检索  
检索        

蝎毒多肽提取物对A549细胞增殖抑制作用机制研究
引用本文:王晓慧,王兆朋,张月英,贾青,王朝霞,张捷,张维东.蝎毒多肽提取物对A549细胞增殖抑制作用机制研究[J].中国中药杂志,2012,37(11):1620-1623.
作者姓名:王晓慧  王兆朋  张月英  贾青  王朝霞  张捷  张维东
作者单位:1. 山东省医学科学院基础医学研究所病理室,山东济南,250062
2. 山东省立医院中心实验室,山东济南,250001
基金项目:国家自然科学基金项目(81073102,30873408);山东省自然科学基金项目(ZR2010HQ003,2009ZRC03044);济南市科学技术发展计划(201004012)
摘    要:目的:观察蝎毒多肽提取物(PESV)对非小细胞肺癌细胞株A549的增殖抑制作用及可能的作用机制。方法:采用噻唑蓝(MTT)法观察不同浓度PESV对A549细胞生长与增殖的影响,下游实验将对数生长期的A549细胞分为阴性对照组、PESV低、中、高剂量组,应用流式细胞术、免疫细胞化学法、Western blot法检测PESV干预后细胞周期及VEGF,HIF-1α和PTEN蛋白表达的变化。结果:MTT结果显示,PESV在一定浓度范围内对A549细胞的增殖活性有明显抑制作用(P<0.01)。流式细胞法、免疫细胞化学法及Western blot法结果显示,PESV干预后能使A549细胞阻滞于G0/G1期,并显著下调HIF-1α,VEGF表达,上调PTEN表达。结论:PESV能够抑制A549细胞的增殖,其作用机制可能与影响血管生成因子VEGF,HIF-1α和PTEN的表达而直接抑制细胞增殖、阻滞细胞周期和抑制血管生成有关。

关 键 词:蝎毒多肽提取物  非小细胞肺癌  血管生成因子  细胞周期  细胞增殖
收稿时间:2011/9/14 0:00:00

Mechanisms for inhibition effects of polypeptide extract from scorpion venom (PESV) on proliferation of A549 cell lines in vitro
WANG Xiaohui,WANG Zhaopeng,ZHANG Yueying,JIA Qing,WANG Zhaoxi,ZHANG Jie and ZHANG Weidong.Mechanisms for inhibition effects of polypeptide extract from scorpion venom (PESV) on proliferation of A549 cell lines in vitro[J].China Journal of Chinese Materia Medica,2012,37(11):1620-1623.
Authors:WANG Xiaohui  WANG Zhaopeng  ZHANG Yueying  JIA Qing  WANG Zhaoxi  ZHANG Jie and ZHANG Weidong
Institution:Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China;Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China;Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China;Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China;Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China;Department of Central Laboratory, Shandong Provincial Hospital, Ji'nan 250001, China;Department of Pathology, Institute of Basic Medcine, Shandong Academy of Medical Science, Ji'nan 250062, China
Abstract:Objective: To investigate the mechanisms for inhibition effects of PESV on proliferation of non-small cell lung cancer cell line A549.Method: MTT was used to observe cell growth and proliferation of A549 at different concentrations of PESV.Flow cytometry(FCM)was applied to analyze cell cycle distribution.Immunocytochemistry and western blot assay was recruited to detect the expression of VEGF,HIF-1α,PTEN after the intervention of PESV.Result: A549 cells may be arrested mainly in G0/G1 phase and cell proliferation was significantly inhibited(P <0.01) after PESV intervention in a certain range of concentration.PESV can significantly reduce the expression of HIF-1α,VEGF and increase the expression of PTEN.Conclusion: PESV can block cell cycle and inhibit angiogenesis directly to inhibit cell proliferation of non-small cell lung cancer cell line A549 mainly through reducing the expression of HIF-1α,VEGF and increasing the expression of PTEN.
Keywords:PESV  non-small cell lung cancer  angiogenesis factor  cell cycle  cell proliferation
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号