芍药内酯苷和芍药苷对慢性束缚应激大鼠神经营养因子以及一氧化氮影响的研究

研究芍药内酯苷、芍药苷对慢性束缚应激肝郁大鼠的作用及机制。以氟西汀及逍遥丸为对照,检测体重,糖水消耗量,并进行旷场行为学实验;格瑞斯比色法检测海马一氧化氮(NO)的含量;酶免法(ELISA)检测海马脑源性神经营养因子(BDNF)的含量;RT-q PCR法检测海马神经元一氧化氮合酶(n NOS)m RNA表达;Western blot法检测海马中n NOS以及BDNF的蛋白表达。与模型组相比,30 mg·kg~(-1)芍药苷、30 mg·kg~(-1)芍药内酯苷明显升高穿行次数(P0.05;P0.01);芍药苷、芍药内酯苷明显降低NO水平(P0.05,P0.01;P0.05,P0.05);30 mg·kg~(-1)芍药苷与30 mg·kg~(-1)芍药内酯苷明显升高BDNF水平(P0.05)。与模型组相比,芍药苷、芍药内酯苷明显降低n NOS m RNA表达(P0.01;P0.05);30 mg·kg~(-1)芍药苷、30 mg·kg~(-1)芍药内酯苷明显升高BDNF蛋白表达(P0.05)。芍药内酯苷、芍药苷为白芍柔肝解郁功效的有效成分,其作用机制与调节海马NO含量以及BDNF的表达有关。

Investigation of effects of albiflorin and paeoniflorin on hippocampal BDNF and NO in chronic restraint stress rats

This paper aimed to investigate the antidepressant-like effects and the mechanism of the albiflorin, paeoniflorin on rats with chronic restraint stress model. Fluoxetine and Xiaoyao group served as the positive control, body weight, sucrose preference test and the open field behavioral experiment were measured, the levels of nitric oxide (NO) in hippocampus were detected by Greg colorimetric method. Furthermore, the levels of brain derived neurotrophic factor (BDNF) in hippocampus were detected by ELISA. Finally, the expressions of nNOS mRNA in hippocampus detected by RT-qPCR, the protein levels of nNOS and BDNF in hippocampus were detected by Western blot. Compared with the model group,the pass counts of paeoniflorin group(30 mg·kg^-1) and albiflorin group(30 mg·kg^-1) were obviously increased(P<0.05,P<0.01). Furthermore, compared with the model group,the levels of NO in paeoniflorin groups(30 mg·kg^-1 and 15 mg·kg^-1) and albiflorin groups(30 mg·kg^-1 and 15 mg·kg^-1) were all significantly decreased(P<0.05,P<0.01; P<0.05, P<0.05). BDNF levels of paeoniflorin group(30 mg·kg^-1) and albiflorin group(30 mg·kg^-1) were obviously increased(P<0.05). Finally, compared with the model group, the expressions of nNOS mRNA of paeoniflorin groups(30 mg·kg^-1 and 15 mg·kg^-1) (P<0.01, P<0.01) and albiflorin groups(30 mg·kg^-1) were significantly decreased(P<0.05). Compared with the model group, the protein exprsssions of BDNF of paeoniflorin group(30 mg·kg^-1) and albiflorin group(30 mg·kg^-1) were obviously increased(P<0.05). Albiflorin and paeoniflorin have the effects of smooth the liver and dispel stagnation, the mechanism has the relevant with adjusting and controlling the expression of NO and BDNF in hippocampus.