西洋参叶20s-原人参二醇组皂苷对犬急性心肌梗死的保护作用

目的 :研究西洋参叶 20s 原人参二醇组皂苷 (PQDS)对犬急性心肌梗死的保护作用。方法 :采用麻醉开胸犬结扎左冠状动脉前降支 (LAD)产生急性心肌梗死模型 ,测定心肌梗死面积 ,血清CK ,LDH活性 ,心肌代谢 ,自由基及冠脉循环等参数。结果 :PQDS 10 ,2 0mg·kg^-1对急性心肌梗死 6h犬 ,能明显缩小心肌梗死面积 ,降低血清CK及LDH活性 ,并明显降低血清FFA及LPO含量 ,提高SOD及GSH-Px活性。亦能明显增加心肌血流量 ,降低冠脉阻力。结论 :PQDS对缺血心肌具有保护作用 ,可能与其纠正心肌缺血时FFA代谢紊乱 ,对抗氧自由基引发的脂质过氧化反应 ,增强体内抗氧化酶活性以及增加心肌供血有关。

Protective Effect of Panax quinquefolium 20s-proto-panaxdiolsaponins on Acute Myocardial Infarction in Dogs

Objective: To study the protective effects of Panax quinquefolium 20s protopanaxdiolsaponins extracted from leaves of P.quinquefolium (PQDS)on acute myocardial infarction(AMI)in dogs. Method:The parameters of myocardial infart size , the serum CK and LDH activity, myocardial metabolism,free radicals and coronary circulation etc were determined by using the model of ligation of LAD in the anaesthetized open chest dogs. Result:In dogs treated with PQDS(in a dosage of 10 and 20 mg·kg^-1 iv infusion),the myocardial infarct size, the activity of serum CK ,LDH and the contents of serum FFA and LPO were decreased,whereas the activity of serum SOD and GSH Px increased markedly.At the same time, myocardial blood flow was increased and coronary vascular resistance decreasedsignificantly. Conclusion:PQDS has protective effect on myocardial ischemia by modifying metabolic dysfunction of FFA, inhibiting oxygen free radicalmediated peroxidation of membrane lipids , enhancing endogenous antioxidase activity and increasing myocardial blood supply.