补气活血药对急性心肌梗死模型大鼠梗死心肌边缘区域新生血管的影响

目的：探讨补气活血药物对急性心肌梗死模型大鼠梗死心肌边缘区新生微血管数目以及VEGF，bFGF蛋白表达的影响。方法：构建大鼠急性心肌梗死动物模型。造模成功后，将大鼠随机分为：假手术组、模型组、丹参-黄芪（1：2）组、丹参-黄芪（1：1）组、川芎-黄芪（1：2）组、丹参组、川芎组、赤芍组、麝香保心丸组，每组5只。各给药组按照13.5 g·kg^-1·d^-1灌胃，每日1次，共给药3周。采用免疫组化SP法检测心肌组织中vWF的表达，计数微血管数目（MVC）；采用Western blotting印迹法检测心肌组织中VEGF，bFGF蛋白的表达。结果：假手术组、模型组、各给药组大鼠梗死心肌边缘区域均可见vWF因子标记染色的新生微血管，假手术组大鼠心肌中新生微血管不明显，模型组大鼠梗死心肌边缘区域可见到少量新生成的微血管，各给药组大鼠梗死心肌边缘区域可见较多新生微血管。模型组与假手术组比较，差异有统计学意义（P<0.05）；各给药组与模型组相比较差异有统计学意义（P<0.05或P<0.01）。假手术组大鼠心肌中的VEGF，bFGF的蛋白表达最少，模型组与之相比较，差异具有统计学意义（P<0.05），各给药组与模型组相比较，VEGF，bFGF的蛋白表达有较显著的增多，差异具有统计学意义（P<0.05或P<0.01）。结论：丹参、川芎、赤芍、丹参-黄芪（1：2）组、丹参-黄芪（1：1）组、川芎-黄芪（1：2）组药物可以促进血管的生成，推测这些药物促进血管生成的机制可能是通过促进VEGF，bFGF的蛋白表达，从而增加VEGF，bFGF的含量以促进新生血管的生成。

Effect of medicines for activating blood and reinforcing Qi on angiogenesis in infarcted myocardium edge area of acute myocardial infarction model in rats

Objective: To study the effect of medicines for activating blood and reinforcing Qi on the number of new micro-vessels and the protein expressions of VEGF and bFGF in the infarcted myocardium edge area of acute myocardial infarction(AMI) model in rats. Method: The AMI model of rats was established. After the successful model establishment, rats were randomly divided into the sham-operated group, the model group, the Danshen-Huangqi(1:2) group, the Danshen-Huangqi(1:1) group, the Chuanxiong-Huangqi(1:2) group, the Danshen group, the Chuanxiong group, the Chishao group and the Shexiang Baoxin pill group, with five rats in each group. Rats in each medicated group were orally administered with drugs as per 13.5 g·kg^-1·d^-1 once everyday for three weeks. The immunohistochemical SP method was adopted to detect the expression of vWF in myocardial tissues, and count the number of micro-vessels (MVC). The protein expression of VEGF and bFGF in myocardial tissues were determined by Western blot. Result: The new micro-vessels stained by vWF factor could be found in the infarcted myocardium edge area of the sham-operated group, the model group and all of medicated groups. The sham-operated group show unobvious new micro-vessels in myocardial tissues. A small amount of new micro-vessels could be seen in the infarcted myocardium edge area of the model group. Whereas a larger number of micro-vessels could be seen in the infarcted myocardium edge area of all of medicated groups. The differences between the sham-operated group and the model group had statistical significance (P<0.05). The differences between each medicated group and the model group had statistical significance as well (P<0.05 or P<0.01). The lowest protein expression of VEGF and bFGF was found in myocardium of the sham-operated group, with the statistical significance compared with the model group (P<0.05). Compared with the model group, each medicated group showed significant increase in the protein expression of VEGF and bFGF, with the statistical significance between them (P<0.05 or P<0.01). Conclusion: The Danshen group, the Chuanxiong group, the Chishao group, the Danshen-Huangqi (1:2) group, the Danshen-Huangqi (1:1) group and the Chuanxiong-Huangqi (1:2) group show the effect in promoting angiogenesis. Their mechanism for promoting angiogenesis may be related to the improvement of the protein expressions of VEGF and bFGF, so as to increase the contents of VEGF and bFGF and promote the angiogenesis of new vessels.