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束花石斛及其相似种的DNA分子鉴别
引用本文:丁小余,徐珞珊,王峥涛,徐红,周开亚.束花石斛及其相似种的DNA分子鉴别[J].中国中药杂志,2002,27(6):407-411.
作者姓名:丁小余  徐珞珊  王峥涛  徐红  周开亚
作者单位:1. 中国药科大学,中药学院,江苏,南京,210038;南京师范大学,生命科学学院,江苏,南京,210097
2. 中国药科大学,中药学院,江苏,南京,210038
3. 南京师范大学,生命科学学院,江苏,南京,210097
基金项目:国家自然科学基金资助项目 (3 0 17114 4)
摘    要:目的 :探讨束花石斛与同属相似种 :玫瑰石斛、喇叭唇石斛、报春石斛、兜唇石斛等植物及药材鉴别的DNA分子标记。方法 :对束花石斛及其相似种进行rDNAITS区序列比较研究。结果 :尽管束花石斛及其相似种的外部形态差异较小 ,但在rDNAITS序列上却存在着显著而稳定的差异。在rDNAITS区域中 ,作者共挑选了15个碱基位点用作鉴别束花石斛及其相似种的DNA标记。结论 :根据rDNAITS区碱基序列差异 ,能准确鉴别束花石斛及其相似种植物及药材。

关 键 词:束花石斛  喇叭唇石斛  报春石斛  兜唇石斛  玫瑰石斛  rDNAITS区序列  DNA序列鉴别
文章编号:1001-5302(2002)06-0407-05
收稿时间:2001/9/11 0:00:00
修稿时间:2001年9月11日

Molecular Authentication of Dendrobium chrysanthum from its Allied Species of Dendrobium
DING Xiao yu ;;XU Luo san ;WANG Zheng tao ;XU Hong ;ZHOU Kai ya.Molecular Authentication of Dendrobium chrysanthum from its Allied Species of Dendrobium[J].China Journal of Chinese Materia Medica,2002,27(6):407-411.
Authors:DING Xiao yu ;;XU Luo san ;WANG Zheng tao ;XU Hong ;ZHOU Kai ya
Institution:Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, Jiangsu, China. dingxynj@263.net
Abstract:Objective: To define molecular characters to distinguish D. chrysanthum from its allied species D. primulinum, D. lituiflorum,D. aphyllum,D. crepidatum. Method: The molecular characteristics of D. chrysanthum and its allied species were compared. The sequences of rDNA ITS regions were exploited to explore the evidence for authentication D. chrysanthum and its allied species. Result: Although the morphological difference was slight, the sequence difference of ITS regions among five rDNAs was obvious and stable. Fifteen sites of ITS region were defined as DNA character to identify D. chrysanthum from the other four allied species. Conclusion: The difference of rDNA ITS sequences can be used to authenticate accurately D. chrysanthum from three allied species of Dendrobium .
Keywords:D  chrysanthum  D  lituiflorum  D  primulinum  D  aphyllum  D  crepidatum  sequences of rDNA ITS region  authentication by DNA sequence
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