| 人参皂苷Rg1对人神经干细胞功能表达的膜片钳研究 |
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| 引用本文： | 姜英虹,李英博,赵香琴,陈笛,姜蓉,王莎莉.人参皂苷Rg1对人神经干细胞功能表达的膜片钳研究[J].中国中药杂志,2012,37(22):3477-3480. |
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| 作者姓名： | 姜英虹 李英博 赵香琴 陈笛 姜蓉 王莎莉 |
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| 作者单位： | 1. 重庆医科大学神经科学研究中心,重庆市神经生物重点实验室,重庆400016
2. 重庆医科大学干细胞与组织工程研究室,重庆,400016 |
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| 基金项目： | 重庆市渝中区科技计划项目(20110310) |
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| 摘 要： | 目的:观察人参皂苷Rg1诱导人神经干细胞(neural stem cells of human,hNSCs)的功能表达.方法:采用全细胞膜片钳技术分析人参皂苷Rg1诱导hNSCs分化7d时,神经元样细胞的膜电生理特性以及钠、钾离子通道的功能表达.结果:人参皂苷Rg1(20 mg·L-1)诱导hNSCs分化7d时,神经元样细胞的膜静息电位为(-45.70 ±2.63)mV,膜电容为(26.89±1.91) pF,膜输入阻抗为(877.51±20.44) MΩ(与对照组相比均P＜0.05)；电压依赖性的快速激活、快速失活的内向Na+电流,检出率50％,平均峰值为(711.48±158.03) pA(与对照组相比P＜0.05)；外向K+电流的主要成分是快速激活快速失活的瞬时外向K+电流和延迟整流外向K+电流,其平均峰值为(1 070.42±177.18) pA(与对照组相比P＜0.05).结论:人参皂苷Rg1可以促进hNSCs功能表达和成熟.
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| 关 键 词： | 人参皂苷Rg1 人神经干细胞 功能表达 膜片钳 |
| 收稿时间： | 2012/2/17 0:00:00 |
Effect of ginsenoside Rg1 on functional expression of human neural stem cells:a patch clamp study |
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| JIANG Ying-hong,LI Ying-bo,ZHAO Xiang-qin,CHEN Di,JIANG Rong and WANG Sha-li.Effect of ginsenoside Rg1 on functional expression of human neural stem cells:a patch clamp study[J].China Journal of Chinese Materia Medica,2012,37(22):3477-3480. |
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| Authors: | JIANG Ying-hong LI Ying-bo ZHAO Xiang-qin CHEN Di JIANG Rong and WANG Sha-li |
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| Institution: | Key Laboratory of Neurobiology of Chongqing, Research Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China;Key Laboratory of Neurobiology of Chongqing, Research Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China;Key Laboratory of Neurobiology of Chongqing, Research Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China;Key Laboratory of Neurobiology of Chongqing, Research Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China;Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China;Key Laboratory of Neurobiology of Chongqing, Research Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, China |
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| Abstract: | |
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| Keywords: | ginsenoside Rg1 human neural stem cell functional expression patch clamp |
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