大孔吸附树脂纯化贯叶金丝桃总黄酮的在线检测研究

目的:以贯叶金丝桃总黄酮的制备为例,设计一种具有在线检测功能的大孔吸附树脂柱色谱系统。方法:经过对常规检测方法的比较分析,选择具有流动检测功能的紫外检测器为在线检测器,使用自制分流器进行连接,设计了大孔树脂分离纯化中药成分的在线检测系统,并用于贯叶金丝桃总黄酮的纯化。结果:在线检测系统操作简单,检测结果准确客观,制备得到的贯叶金丝桃总黄酮含量可达到90%以上。结论:该技术为中药提取物色谱纯化的工业化生产提供了简单易行、稳定可靠的在线检测方法,为中药纯化自动化生产奠定基础。

Purification of total flavonoid glycosides from Hypericum perforatum by macroporous adsorption resin with on-line detection system

Objective:To design a macroporous resin chromatography system with on-line detector which can be used to purifytotal flavonoid glycosides from Hypericum perforatum.Method:Compared with the present detection methods,the ultraviolet detector which can be applied to flow liquid,and a self-made shunt were applied to design the on-line detection system.This system was successfully applied to purify the total flavonoid glycosides from H.perforatum.Result:This on-line detection system was simple and feasible,and the results were accurate and reliable.The purity of total flavonoid glycosides from H.perforatum.separated by this system was higher than 90%.Conclusion:This technique provides a simple and reliable on-line detection method for industrial chromatography purification of TCM extracts,which is the basis of automatic manufactory of TCM purification.