真菌诱导子对人参毛状根皂苷生物合成和生长的影响

目的 :考察真菌诱导子对人参毛状根的生长和人参皂苷生物合成影响。方法 :提取黄瓜炭疽病菌Colletorichumlagnarinm、青菜炭疽病菌Phomafiltrate、棉花枯萎病菌Fusariumoxysponum、黑曲霉Asperillusniger中的真菌诱导子与人参毛状根共培养 ,应用HPLC及比色法对培养物中总苷及几种单体皂苷进行分析测定。结果 :真菌诱导子不但能影响人参毛状根总苷的合成量 ,也能使某些单体皂苷消失或增加 ,如培养液中黑曲霉多糖诱导子增加到 20mg·L^-1浓度时 ,使总苷含量增加到 3.649% ,而单体皂苷中Rg₁和Re未检出 ,Rg₂ 和Rb₁的含量则有明显增加 ,并可促进人参毛状根的生长。结论 :真菌诱导子对人参毛状根某些皂苷的合成具有特异性 ,同时也影响人参毛状根的生物量。培养过程中通过外源性诱导子的添加 ,有利于人参毛状根次生代谢产物的定向积累。

Studies on influence of funal elicitor on hairy root of Panax ginseng biosynthesis ginseng saponin and biomass

Objective:To study influence of fungal elicitors on the biomass and ginseng saponin biosynthesis of hairy roots of Panax ginseng (HRPG). Method: Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV spectrophotometry and RP HPLC. Result: Fungal elicitors coula not only can influence on HRPG biomass and total ginseng sponin, but also improve or decrease some monomeric sponin. The total ginseng sponin could be increased to 3.649% but Rg₁ and Re could not be detected when A. niger elicitors wss 20 mg·L^-1 in the culture fluid. Conclusion:Fungal elicitor has specificity influence on secondary metabolite of HRPG. HRPG can biosynthesize specially active component by using specific fungal elicitor is used.