| 人参农家类型的AFLP指纹研究 |
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| 引用本文： | 马小军,汪小全,肖培根,洪德元.人参农家类型的AFLP指纹研究[J].中国中药杂志,2000,25(12):707-710. |
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| 作者姓名： | 马小军 汪小全 肖培根 洪德元 |
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| 作者单位： | [1]中国医学科学院,北京 [2]中国科学院植物研究所系统与进化植物学开放实验室,北京 |
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| 摘 要： | 目的：为了获得更多人参DNA指纹信息，寻找鉴定5个农家类型DNA特征指纹的线索，更有效的比较农家类型之间的遗传差异。方法：采用扩增酶切片段多态性(AFLP)方法研究5种农家类型。结果和结论：5种人参农家类型多态位点仅4.6%，说明各农家类型之间的遗传差异性很小，但长脖的AFLP有相对较高的多态性，说明长脖类型内部有更多的杂合态个体，更接近野生人参。
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| 关 键 词： | 人参 农家类型 AFLP 指纹 |
| 收稿时间： | 2000/8/18 0:00:00 |
A Study on AFLP Fingerprinting of Land Races of Panax ginseng L. |
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| MA Xiao jun ,WANG Xiao quan ,XIAO Pei gen ,HONG De yuan.A Study on AFLP Fingerprinting of Land Races of Panax ginseng L.[J].China Journal of Chinese Materia Medica,2000,25(12):707-710. |
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| Authors: | MA Xiao jun WANG Xiao quan XIAO Pei gen HONG De yuan |
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| Institution: | Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094, China. |
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| Abstract: | Objective:To obtain more information on DNA fingerprintings of five land races of Chinese ginseng, namely, Damaya (DMY), Changbo (CB), Yuanbangyuanlu (YBYL) and Huangguo (HG). Mothods:The five land races were detected by amplified restriction fragment polymorphism (AFLP) markers with 11 combined primers (M2,M3,M16,M20,M53,M56,M57,M68,M69,M72,M84 in Mse I).Result and Conclusion:Only 4.6% polymorphic sites was found. It was further verified that only a little diversity existed amoung the land races. The polymorphic sites of CB were much more than those of the others, which suggests that there are more heterozygotes in CB populations, and it is closer to wild ginseng than the others. |
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| Keywords: | Panax ginseng land races AFLP fingerpint |
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