山楂叶抗大鼠PMN呼吸爆发谱效关系研究

目的：研究山楂叶HPLC指纹图谱与其抗大鼠中性粒细胞(PMN)呼吸爆发活性之间的相互关系。方法：分别对不同种山楂叶HPLC指纹图谱的各指纹峰进行抗大鼠PMN呼吸爆发药效实验，建立各指纹峰峰面积与药效之间关系(峰效关系)的数学模型，再通过数学、化学计量学、计算机编程与模拟等手段将山楂叶的HPLC指纹图谱翻译成生物活性指纹图谱(包括药效指纹图谱和效价指纹图谱)。结果：建立了山楂叶抗大鼠PMN呼吸爆发谱效关系，利用该关系对14个山楂叶样品进行活性预测，其预测值与实际值呈显著正相关，r=0.968(P<0.01)。结论：建立了一种融化学分析与生物活性评价为一体的综合质量评价体系，为中药指纹图谱研究提供了一条新的思路。

Relationship between HPLC fingerprint chromatogram and inhibitory effecton respiratory burst of rat PMN of leaves of crataegus

Objective: To study the relationship between HPLC fingerprint chromatogram and inhibitory effect on respiratory burst of rat PMN of leaves of crataegus L. Method: HPLC fingerprint peaks of different species of hawthorn leaves were isolated and used for the effective experiment on the respiratory burst of rat PMN. The mathematic models of the relationship between the area and the effect of fingerprint peaks were established. According to the mathematic models, the HPLC fingerprint were change into bioactive fingerprint (include effective fingerprint and potency fingerprint) with the helps of mathematics, chemometrics, computer program simulation and etc. Result: The chromatogram-effect relationship of leaves of crataegus. on respiratory burst of rat PMN was established. According to this relationship, the activities of fourteen samples of leaves of crataegus. were forecasted. It was positive correlation between the expected value and the practical value. And the correlation coefficients was 0.968 (P<0.01). Conclusion: An all-around evaluative system, which includes not only chemical identification but also effective evaluation for traditional Chinese medicine was established. It will provide a new idea for study on fingerprint chromatogram of traditional Chinese medicine.