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Smashing Tissue Extraction and HPLC Determination of Active Saponins from Different Parts of Panax notoginseng
Authors:SHI Sheng-ming  LIU Yan-ze  TAI Wen  CHEN Chang-qing and ZHAO Yu-qing
Institution:SHI Sheng-ming1,2,LIU Yan-ze3,4,TAI Wen2,CHEN Chang-qing2,ZHAO Yu-qing1,5 1.Shenyang Pharmaceutical University,Shenyang 110006,China 2.Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China 3.Bio-Organic and Natural Product Laboratory,MRC 311,McLean Hospital/Harvard Medical School,115 Mill Street,Belmont,MA 02478,USA 4.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China 5.Key Laboratory of Structure-Based Drug Design and Discovery,Ministry of Education,Shenyang Pharmaceutical University,Shenyang 110016,China
Abstract:ObjectiveTo optimize the extraction technology used for extracting active saponins from the roots, fibrous roots, basal part of stems, root verrucae, fruits, flowers, stems, and leaves of Panax notoginseng based on the contents of ginsengsides Rg1, Rb1, and notoginsengside R1 as evaluation indexes.MethodsDifferent parts of P. notoginseng were extracted by smashing tissue extraction (STE), ultrasound extraction, and reflux extraction. The contents of ginsengsides Rg1, Rb1, and notoginsengside R1 in 24 kinds of extracts were determined by HPLC-UV. Hypersil C18 column (200 mm × 4.6 mm, 5 μm) and acetonitrile-warter (20:80 for 30 min→45:55 for 18 min→70:30 for 2 min→80:20 for 10 min→100:0) were used; UV detector was set at 203 nm; The flow rate was set at 1.0 mL/min.ResultsSTE was the most efficient technology with the highest yield of active saponins among the three tested extraction technologies.ConclusionSTE is a fast, effective, and economical method to extract the active saponins from different parts of P. notoginseng. It could significantly shorten the extraction time and simplify the determination of the pre-processing work on identifying P. notoginseng. Such quick and effective extraction provides a powerful tool for analyzing P. notoginseng in the future.
Keywords:ginsengside Rb1  ginsengside Rg1  HPLC  notoginsengside R1  Panax notoginseng  smashing tissue extraction
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