| 白藜芦醇对K562/ADM细胞促凋亡作用研究 |
| |
| 引用本文： | 温志震,魏虎来,苏海翔,郝春燕.白藜芦醇对K562/ADM细胞促凋亡作用研究[J].中药药理与临床,2010(1). |
| |
| 作者姓名： | 温志震 魏虎来 苏海翔 郝春燕 |
| |
| 作者单位： | 甘肃省第二人民医院血液肿瘤科;兰州大学医学实验中心;兰州大学基础医学院生化与分子生物学教研室; |
| |
| 摘 要： | 目的:探讨白藜芦醇诱导K562/ADM细胞凋亡的效应与细胞活性氧水平的关系。方法:应用噻唑蓝(MTT)比色法、光镜、电镜和流式细胞术(FCM)检测细胞凋亡;FCM测定细胞内活性氧(ROS)水平。结果:白藜芦醇显著抑制K562/ADM细胞增殖,形态学呈现典型的凋亡改变;FCM分析显示S期细胞数明显增多,出现S/G2期阻滞;ROS水平升高。结论:白藜芦醇诱导K562/ADM胞凋亡与细胞内活性氧水平升高有关。
|
| 关 键 词： | 白藜芦醇 白血病 细胞凋亡 活性氧 |
Correlation of reactive oxygen species and apoptosis induced by resveratrol in drug-resistant K562/ADM cell |
| |
| Wen Zhizhen,WeI Hulai,Su Haixiang,Hao Chunyan.Correlation of reactive oxygen species and apoptosis induced by resveratrol in drug-resistant K562/ADM cell[J].Pharmacology and Clinics of Chinese Materia Medica,2010(1). |
| |
| Authors: | Wen Zhizhen WeI Hulai Su Haixiang Hao Chunyan |
| |
| Institution: | Wen Zhizhen1,WeI Hulai2,Su Haixiang2,Hao Chunyan3(1 The Second People\'s Hospital of Gansu Province,Lanzhou 730000,2 Lanzhou University,Lanzhou 730000) |
| |
| Abstract: | Objective:To investigate the apoptosis of K562/ADM cells induced by resveratrol and the influence of intracellular reactive oxygen species(ROS)level.Methods:MTT assay,light and electronic microscopy and cell cycle analysis were used to examine apoptosis in K562/ADM cells.Intracellular ROS level was measured with flow cytometry(FCM).Results:Resveratrol inhibited growth of K562/ADM cells.Morphological changes typical of apoptosis were observed through light and electronic microscopy.Cell cycle analysis indica... |
| |
| Keywords: | resveratrol leukemia apoptosis reactive oxygen species(ROS) |
| 本文献已被 CNKI 等数据库收录！ |
