| 黄芪甲苷对人外周血内皮祖细胞的保护作用 |
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| 引用本文： | 黄鑫,郭力恒,王磊,黄健安,张敏州.黄芪甲苷对人外周血内皮祖细胞的保护作用[J].中药药理与临床,2012(2):26-29. |
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| 作者姓名： | 黄鑫 郭力恒 王磊 黄健安 张敏州 |
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| 作者单位： | 广东省中医药科学院;广东省中医院重症医学科;广东省中西医结合医院 |
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| 基金项目： | 国家自然科学基金,编号81173439 |
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| 摘 要： | 目的:探讨黄芪甲苷对人外周血内皮祖细胞的保护作用。方法:用密度梯度离心法获取单个核细胞(MNCs),培养7天后,收集贴壁细胞,采用多波长激光共聚焦显微镜(laserscanningeonfoealmicroscope,LSCM)鉴定后将细胞随机分成:(l)对照组;(2)辛伐他汀对照组;(3)黄芪甲苷组。24h后采用流式细胞仪检测EPCs的增殖周期;MTT比色法、黏附能力实验检测内皮祖细胞(En-dothelialprogenitoreells,EPCs)的增殖能力、黏附能力。结果:黄芪甲苷在10-1mmol/L～10-10mmol/L的浓度范围中均有效,在黄芪甲苷10-9mmol/L浓度时,EPCs的增殖力最强,黄芪甲苷能明显增强EPCs的黏附、增殖能力。结论:黄芪甲苷能够增强EPCs的生物学功能,对EPCs有保护作用。
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| 关 键 词： | 内皮祖细胞 黄芪甲苷 增殖 黏附 |
Protection of Astragaloside on the peripheral blood endothelial progenitor cells |
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| Huang Xin,Guo Liheng,Wang Lei,Huang Jian’an,Zhang Minzhou.Protection of Astragaloside on the peripheral blood endothelial progenitor cells[J].Pharmacology and Clinics of Chinese Materia Medica,2012(2):26-29. |
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| Authors: | Huang Xin Guo Liheng Wang Lei Huang Jian’an Zhang Minzhou |
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| Institution: | 1,2(1 Guangdong Provincial Academy of Chinese Medical Sciences,Guangzhou 510006;2 Guangdong Provincial Hospital of Traditional Chinese Medicine ICU,Guangzhou 510120;3 Guangdong Hospital of Integrated Traditional Chinese and Western Medicine,Foshan 528200) |
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| Abstract: | Objective: To explore the effects and mechanisms of astragaloside mobilizing peripheral blood endothelial progenitor cells.Methods: Peripheral blood mononuclear cells(PB-MCs) were isolated by Ficoll density gradient centrifugation,and EPCs were identified by laserscanningeonfoealmicroscope(LSCM).Adherent cells were collected after seven days’ incubation and randomly divided into 3 groups(Control,simvastatin,astragaloside).The adhesion activity was determined by cell counts.The proliferation was determined by MTT assay and cell proliferation cycle analysis assay.Result: Compared with the control group,the numbers of EPCs adhesion,proliferation and cell proliferation cycle in astragaloside group and simvastatin group were significant(P < 0.01).The proliferative capacities of EPCs were improved in astragaloside group from 10-1mmol / L to 10-10mmol / L,significantly in 10-9mmol / L group.Conclusion: astragaloside has a significant protective effect of EPC. |
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| Keywords: | Astragaloside endothelial progenitor cells proliferation adhesion |
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