| 基于 LC Chip Q-TOF MS/MS 的非标记比较策略研究丙二酰黄芪皂苷 I 抗白血病 U937 细胞的差异蛋白质组(英文) |
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| 引用本文： | 彭咏波,周萍,楚楚,刘鄂湖,闻晓东,刘群,陈君,高宁,齐炼文,李萍.基于 LC Chip Q-TOF MS/MS 的非标记比较策略研究丙二酰黄芪皂苷 I 抗白血病 U937 细胞的差异蛋白质组(英文)[J].中国天然药物,2011(4):305-316. |
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| 作者姓名： | 彭咏波 周萍 楚楚 刘鄂湖 闻晓东 刘群 陈君 高宁 齐炼文 李萍 |
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| 作者单位： | 中国药科大学天然药物活性物质与功能国家重点实验室;中国人民解放军第三军医大学药学院;中国药科大学生药学教研室; |
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| 基金项目： | supported by Program for Changjiang Scholars and Innovative Research Team in University (No. IRT0868); National Science and Technology Major Project 'Creation of Major New Drugs' from China (No. 2009ZX09502-020)~~ |
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| 摘 要： | 目的:研究丙二酰黄芪皂苷 I 抗白血病 U937 细胞增殖的差异表达蛋白质组。方法:基于 LC Chip Q-TOF 系统建立和优化了一种非标记蛋白质组比较方法-多肽色谱峰面积。方法学研究表明,该方法操作简单、方便,具有较高的重复性和宽动力学范围。然后,将建立的优化方法组合归一化计算应用于新化合物丙二酰黄芪皂苷 I 抗白血病 U937 细胞增殖的差异蛋白质组表达研究。结果:分析结果表明,2 倍以上表达差异的蛋白质 15 个,其中 6 个蛋白质的表达差异获得了 Western blotting 确证。此外,通过差异蛋白质的相互作用分析,PARP1、Caspase-3 和-9 的 Western blot 检测及 Caspase 抑制剂实验,我们发现 Caspase激活在丙二酰黄芪皂苷 I 抗白血病 U937 细胞增殖效应中起重要作用。结论:通过该优化的非标记蛋白质组比较方法,找到了一些与丙二酰黄芪皂苷 I 抗白血病 U937 细胞增殖密切相关的功能蛋白质,为深入开展其分子机理研究奠定了基础。
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| 关 键 词： | LC Chip Q-TOF 非标记 多肽色谱峰面积 丙二酰黄芪皂苷 I 细胞增殖 |
Proteomic Analysis for Malonylastragaloside I in U937 Leukemia Cells by Modified Label-free Quantitative Strategy with LC Chip Q-TOF MS/MS |
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| PENG Yong-Bo,ZHOU Ping#,CHU Chu,LIU E-Hu,WEN Xiao-Dong,LIU Qun,CHEN Jun,GAO Ning,QI Lian-Wen,LI Ping, State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing ,China.Proteomic Analysis for Malonylastragaloside I in U937 Leukemia Cells by Modified Label-free Quantitative Strategy with LC Chip Q-TOF MS/MS[J].Chinese JOurnal of Natural Medicines,2011(4):305-316. |
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| Authors: | PENG Yong-Bo ZHOU Ping# CHU Chu LIU E-Hu WEN Xiao-Dong LIU Qun CHEN Jun GAO Ning QI Lian-Wen LI Ping State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China |
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| Institution: | PENG Yong-Bo1,ZHOU Ping1#,CHU Chu1,LIU E-Hu1,WEN Xiao-Dong1,LIU Qun1,CHEN Jun1,GAO Ning3,QI Lian-Wen1,2*,LI Ping1,2* 1 State Key Laboratory of Natural Medicines,China Pharmaceutical University,Nanjing 210009,China,2 Department of Pharmacognosy,3 Department of Pharmacognosy,School of Pharmacy,3rd Military Medical University,Chongqing 400038 |
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| Abstract: | AIM:To study the differentially-expressed proteins for malonylastragaloside I in human leukemia U937 cells.METHODS:A suitable protein quantitative mode of label-free technique was established by LC Chip Q-TOF.It was found that the mode of peptide spectral intensity based on label-free quantification strategy of LC Chip Q-TOF showed high reproducibility,wide dynamic range,as well as simplicity and convenience.The optimized quantification mode of the peptide spectral intensity,normalization algorithm was appl... |
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| Keywords: | LC Chip Q-TOF Label-free Peptide spectral intensity Malonylastragaloside I Cell proliferation |
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