| 乌贼墨酶解肽提取方法及其抗肿瘤活性(英文) |
| |
| 引用本文： | 丁国芳,黄芳芳,杨最素,郁迪,杨永芳.乌贼墨酶解肽提取方法及其抗肿瘤活性(英文)[J].中国天然药物,2011(2):151-155. |
| |
| 作者姓名： | 丁国芳 黄芳芳 杨最素 郁迪 杨永芳 |
| |
| 作者单位： | 浙江海洋学院食品药学学院医学院; |
| |
| 基金项目： | supported by the Department of Science and Technology of Zhejiang Province,the Major program of Department of Science and Technology of Zhejiang Province(№. 2010C13009); Zhejiang Provincial Natural Science Foundation of China (No.Y2100805); Scientific and Technological planing of zhoushan(No.092016); the Department of Finance and Ministry of Education (graduate student’s innovation research plan) (No. YK2008072)~~ |
| |
| 摘 要： | 目的:用蛋白酶酶解乌贼墨,经纯化获得寡肽,并对其进行抗肿瘤活性研究。方法:通过胰蛋白酶酶解获得乌贼墨酶解物,将酶解液用超滤、G-25分子筛凝胶层析进行分离纯化,高效液相进行纯度检测。分离纯化得到的寡肽进行氨基酸序列检测,并用CCK-8检测寡肽对DU-145细胞增殖的影响和HE染色方法观察其对细胞形态的影响。结果:乌贼墨寡肽的N端氨基酸序列为:Gln-Pro-Lys,得率为1.82%。将寡肽进行抗肿瘤活性研究,发现寡肽对DU-145细胞有明显的增殖抑制效应,且有浓度依赖性。HE染色结果显示了寡肽使细胞形态发生不规则变化,失去原有的恶性表型。结论:用酶解方法制得的乌贼墨寡肽有明显的抗肿瘤活性。
|
| 关 键 词： | 乌贼墨 酶解 寡肽 抗肿瘤 NGln-Pro-Lys |
Anticancer Activity of an Oligopeptide Isolated from Hydrolysates of Sepia Ink |
| |
| DING Guo-Fang,HUANG Fang-Fang,YANG Zui-Su,YU Di,YANG Yong-Fang School of Food , Pharmacy,Zhejiang Ocean University,Zhoushan ,China.Anticancer Activity of an Oligopeptide Isolated from Hydrolysates of Sepia Ink[J].Chinese JOurnal of Natural Medicines,2011(2):151-155. |
| |
| Authors: | DING Guo-Fang HUANG Fang-Fang YANG Zui-Su YU Di YANG Yong-Fang School of Food Pharmacy Zhejiang Ocean University Zhoushan China |
| |
| Institution: | DING Guo-Fang,HUANG Fang-Fang,YANG Zui-Su,YU Di,YANG Yong-Fang School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000,China |
| |
| Abstract: | AIM: To investigate the antitumor effect of peptides from Sepia ink by enzymatic hydrolysis. METHODS: Sepia ink was hydrolyzed with trypsin to prepare peptides; hydrolysates were isolated by ultrafiltration and purified using G-25 gel filtration. The purity of the sepia ink oligopeptides was demonstrated by HPLC and its peptide sequence was detected. Then CCK-8 method was employed to measure cellular viability, and HE staining was used to detect cell morphological change. RESULTS: An oligopeptide was obtain... |
| |
| Keywords: | Sepia ink Enzymatic hydrolysis Oligopeptide Antitumor N Gln-Pro-Lys |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
