高效液相色谱-串联质谱法分析破骨风中裂环环烯醚萜苷类成分

目的：利用高效液相色谱-电喷雾串联质谱法（HPLC—DAD—ESI—MS^2）分析了破骨风中裂环环烯醚萜苷类成分。方法：采用该方法得到了破骨风中裂环环烯醚萜苷类成分的紫外（UV）色谱图、总离子流（TIC）色谱图和萃取离子（EIC）色谱图，以及相应色谱峰的紫外光谱图和EIC／MS^2的质谱图，结合文献对其进行解析。结果：鉴别出破骨风中6个裂环环烯醚萜苷类成分，分别为Jasminoside，Jaslanceoside A，Jaslanceoside B，Jaslanceoside C，Jaslanceoside D和Jaslanceoside E；同时发现还有4个未知结构裂环环烯醚萜苷类化合物存在，并对它们的结构进行了推测。结论：高效液相色谱-电喷雾串联质谱法可以用于分析破骨风中裂环环烯醚萜苷类成分。

Analysis of Secoiridoid Glucosides in Jasminum lanceolatium Roxb. by HPLC-MS

AIM： To develop high performance liquid chromatography-electrospray ionization-mass spectrometry （HPLC-ESI- MS） for the analysis and identification of secoiridoid glucosides in Jasminum lanceolarium Roxb. METHOD： The ultraviolet （UV） chromatogram, total ion current chromatogram （TIC） and extracted ion chromatogram （EIC） of the sample and ESI/MS/MS spectra of the peaks in the chromatograms were obtained in order to identify the structure of secoiridoid glucosides. RESULT： The six identified secoiridoid glucosides were Jasminoside, Jaslanceoside A, Jaslanceoside B, Jaslanceoside C, Jaslanceoside D and Jaslanceoside E. The structures of another four unknown secoiridoid glucosides were speculated. CONCLUSION： The developed simple and rapid method was useful to isolate the new secoiridoid glycoside from Jasminum lanceolarium quickly and to authenticate the species of the herb.