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| 山茱萸免疫活性部位F-1C的免疫抑制作用 | |
| 引用本文： | 吕晓东,杨胜,齐春会,张永祥,茹祥斌,周文霞,吴禾,赵毅民.山茱萸免疫活性部位F-1C的免疫抑制作用[J].中国天然药物,2004,2(1):50-54. |
| 作者姓名： | 吕晓东 杨胜 齐春会 张永祥 茹祥斌 周文霞 吴禾 赵毅民 |
| 作者单位： | 1. 军事医学科学院毒物药物研究所中药和神经免疫药理研究室,北京,100850;解放军305医院药剂科,北京,100017 2. 军事医学科学院毒物药物研究所中药和神经免疫药理研究室,北京,100850 3. 军事医学科学院毒物药物研究所天然药物化学研究室,北京,100850 |
| 基金项目： | 国家重点基础研究发展规划项目资助课题(No .G19990 5 44 0 1) |
| 摘 要： | 目的：研究山茱萸免疫活性部位F-1C对小鼠免疫反应的抑制作用。方法：运用淋巴细胞增殖反应、混合淋巴细胞反应、溶血空斑实验和流式细胞技术观察F-1C体外应用和口服对正常小鼠免疫反应和脾脏T细胞亚群的影响。结果：F-1C在10-200μg/ml剂量范围内无明显细胞毒性，但对小鼠ConA诱导的脾细胞增殖反应、混合淋巴细胞反应和脾细胞抗体生成反应具有明显的抑制作用，对LPS诱导的裸鼠脾细胞增殖反应呈剂量依赖性的促进作用。连续7天口服给予40和80mg/kg，明显抑制脾细胞抗体生成反应和脾细胞自发增殖反应，对脾脏T细胞亚群CD4^ 细胞数目无明显影响，但明显升高CD8^ 细胞百分数，使CD4^ ／CD8^ 比值降低。同时给药组小鼠T抑制细胞(Ts)的抑制功能增强。结论：F-1C对正常小鼠免疫反应具有明显的抑制作用，增加Ts细胞数量并增强Ts细胞抑制功能可能是其发挥免疫抑制作用的途径之一。 |
| 关 键 词： | 山茱萸 活性部位 F-1C 免疫抑制 中药 |
| 文章编号： | 1672-3651(2004)01-0050-05 |
| 修稿时间： | 2003年10月15 |
Pharmacological Study on Immunosuppressive Fractions F-1C from Fructus corni |
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| LU Xiao Dong ,YANG Sheng ,QI Chun Hui ,ZHANG Yong Xiang ,RU Xiang Bin ,ZHOU Wen Xia ,WU He ,ZhAO Yi Min.Pharmacological Study on Immunosuppressive Fractions F-1C from Fructus corni[J].Chinese JOurnal of Natural Medicines,2004,2(1):50-54. | |
| Authors: | LU Xiao Dong YANG Sheng QI Chun Hui ZHANG Yong Xiang RU Xiang Bin ZHOU Wen Xia WU He ZhAO Yi Min |
| Institution: | LU Xiao Dong 1,3,YANG Sheng 1,QI Chun Hui 1,ZHANG Yong Xiang 1,RU Xiang Bin 1,ZHOU Wen Xia 1,WU He 2,ZhAO Yi Min 2 1 Department of Neuroimmunopharmacology of Traditional Chinese Medicine,Beijing Institute of Pharmacology and Toxicology, 2 Department of Natural Medicines,Beijing Institute of Pharmacology and Toxicology,Beijing 100850, 3 Department of Pharmacy,305 Hospital of People's Liberation Army,Beijing,100017 |
| Abstract: | AIM:To investigate the immunosuppressive effect of F 1C,a fraction from the \%Cornus officinals\%, which is one component of Liu Wei Di Huang (LW) decoction. METHOD: The effects of F 1C on immune response were evaluated \%in vitro\% and \%in vivo\%. RESULT: F 1C showed strong inhibiting effect on antibody production response and proliferation of lymphocyte, during concentration range without cytotoxinic effect in vitro. Oral administration of F 1C also significantly inhibited antibody production response and splenocyte proliferation response. F 1C showed no influence on the number of CD 4 splenocyte, however, it increased the number of CD 8 splenocyte and decreased the ratio of CD 4 /CD 8 , suggesting that F 1C increased the quantity of Ts cell. The inhibition function of Ts was also enhanced by F 1C. CONCLUSION: F 1C possesses potent immunosuppressive effect. Enhancing the activity and quantity of Ts cell is one possible underlying mechanism. |
| Keywords: | Fructus Corni Immunosuppressive Active fraction |
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