大孔树脂富集纯化披针新月蕨(Abacopteris penangiana)中的黄酮类成分(英文)

目的:研究使用大孔树脂富集纯化披针新月蕨根茎中的黄酮类成分。方法:通过静态吸附及解吸附试验筛选最优型号树脂;动态吸附、解吸附试验和正交试验优化树脂最大上样量、最佳上样浓度、最佳洗脱醇浓度、吸附流速、解析流速、水洗体积和醇洗体积等工艺参数;采用最佳参数富集纯化黄酮类成分后,HPLC分析和ABTS(2,2′-azinobis-3-ethylbenzothia-zoline-6-sulphonic acid)抗氧化试验比较样品经树脂处理前后的差别。结果:优化大孔树脂富集纯化披针新月蕨黄酮类成分的条件为:上样浓度2.86mg·mL1,醇洗浓度70%,吸附流速1mL·min1,解吸流速2mL·min1,水洗体积和醇洗体积均为5倍柱体积。样品经大孔树脂处理后黄烷-4-醇类黄酮含量明显增加。结论:样品经大孔树脂处理后黄酮含量由21.85%增加到63.12%,表明大孔树脂可以应用于披针新月蕨中黄酮成分的富集纯化过程。

Enrichment and purification of flavones from rhizomes of Abacopteris penangiana by macroporous resins

AIM: To investigate the enrichment and purification of flavones from the rhizomes of Abacopteris penangiana (RAP) by macroporous resins. METHODS: Static adsorption and desorption tests were performed to select the appropriate resin. The kinetic adsorption and desorption experiments were carried out on selected HPD500 resin to optimize the separation process of flavones. Additionally, the effects of four parameters including adsorption flow rate, elute flow rate, volume of water and ethanol solution for elution were explored by a L4/3 orthogonal experiment. Finally, the ABTS (2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities of samples before and after being treated by HPD500 were compared. RESULTS: The results showed that the optimal parameters were initial concentration of 2.86 mg·mL-1, elute solution of 70% ethanol, absorb flow rate of 1 mL·min-1, elute flow rate of 2 mL·min-1, 5 BV of water for elution and 5 BV of ethanol solution for elution. CONCLUSION: The content of flavones is above 60% in RAP after being treated by HPD500, indicating that macroporous resins could be successfully applied to enrich and purify flavones in RAP.