益肾蠲痹丸对类风湿性关节炎的药效学研究

目的：使用弗氏完全佐剂制作大鼠关节炎模型，评价益’肾蠲痹丸对类风湿性关节炎的药效。方法：使用弗氏完全佐剂造模后，测量益肾蠲痹丸对对侧足爪肿胀和脏器指数的影响：ELISA法检测其对血清肿瘤坏死因子Ⅱ（TNF-Ⅱ），白介素1β（IL—1β），白介素-6（IL-6）的影响；分光光度法测量总一氧化氮合酶（tNOS）和诱导型一氧化氮合酶（iNOS）活性：并用Westernblot法检测益肾蠲痹丸对凋亡蛋白Bcl-2表达的影响。结果：益肾蠲痹丸可有效抑制大鼠佐剂性关节炎所引起的足跖肿胀，且对胸腺脾脏指数无明显影响。益肾蠲痹丸可有效抑制血清中TNF-Ⅱ，IL-1β，IL-6含量：抑制血清中iNOS活性；下调Bcl-2在滑膜组织中的表达。结论：益肾蠲痹丸的抗类风湿关节炎的作用与其调节主要炎症因子，下调炎症部位Bcl-2蛋白表达有关。

Effects of Yishen Juanbi(YJB) Pill on Experimental Rheumatoid Arthritis

AIM： To investigate the effects of Yishen Juanbi （YJB） on rheumatoid arthritis （RA）, using Freund＇s complete in- duced adjuvant arthritis （AA） in rat model. METHODS： AA was evaluated by measuring paw swelling and rats＇ secondary organ indexes. The level of serum tumor necrosis factor alpha （TNF-α）, interleukin-1β （IL-1β） and interleukin-6 （IL-6） in rats were further measured by enzyme linked immunosorbent assay （ELISA）. Also, total nitric oxide synthase （tNOS） and inducible nitric oxide synthase （iNOS） were assayed in serum using nitric oxide synthase kits by spectrophotometry. Antiapoptotic Bcl-2 was examined by Western blot. RESULTS： The administration of YJB significantly inhibited the swelling of the adjuvant-non-injected footpad of AA rats while without significant effect on rats＇ secondary organ indexes. YJB significantly decreased the production of serum TNF-α, IL-1β, IL-6, iNOS and over-production of Bcl-2 expression in synovium. CONCLUSION： YJB possesses anti-rheumatic activity by modulating the main anti-inflammatory factors while down-regulating antiapoptotic Bcl-2 in AA.