首页 | 本学科首页   官方微博 | 高级检索  
检索        

五苓散对高尿酸血症小鼠降尿酸及肾保护机制的研究(英文)
引用本文:丁晓琴,潘颖,王星,马玉香,孔令东.五苓散对高尿酸血症小鼠降尿酸及肾保护机制的研究(英文)[J].中国天然药物,2013(3):214-221.
作者姓名:丁晓琴  潘颖  王星  马玉香  孔令东
作者单位:南京大学医药生物技术国家重点实验室,南京210093
基金项目:supported by the National Natural Science Foundation of China(Nos.81025025,81001671,and J1103512); Natural Science Foundation of Jiangsu Province(No.BK2010365)~~
摘    要:目的:研究五苓散对高尿酸血症小鼠降尿酸和肾保护作用并探索其可能的作用机制。方法:在氧嗪酸钾连续7天诱导小鼠产生高尿酸血症模型同时每天灌胃给予不同剂量五苓散(每组10只),并以别嘌呤醇作为阳性药对照。分别测定每组动物血清尿酸、肌酐及尿液尿酸、肌酐等水平并计算其尿酸排泄分数。采用RT-PCR和Westernblot方法分别测定小鼠肾脏尿酸盐重吸收转运体1(mURAT1)、葡萄糖转运体9(mGLUT9)、有机阴离子转运体1(mOAT1)、有机阳离子转运体1和2(mOCT1、mOCT2)及肉毒碱转运体2(mOCTN2)mRNA及蛋白表达水平。结果:与模型组比较,五苓散显著降低高尿酸血症小鼠血清尿酸与肌酐水平,促进尿酸和肌酐排泄,提高尿酸排泄分数,呈现出促进肾脏尿酸排泄与肾保护作用。五苓散显著下调高尿酸血症动物肾脏mURAT1、mGLUT9mRNA及蛋白表达水平,并上调mOAT1、mOCT1、mOCT2以及mOCTN2mRNA及蛋白表达水平。结论:这些结果表明五苓散可介导肾脏有机离子转运体表达以促进高尿酸血症和肾功能异常动物尿酸排泄并发挥其肾保护作用。

关 键 词:五苓散  高尿酸血症  尿酸盐重吸收转运体1  葡萄糖转运体9  有机阴离子转运体1  有机阳离子转运体1和2  肉毒碱转运体2

Wuling San ameliorates urate under-excretion and renal dysfunction in hyperuricemic mice
DING Xiao-Qin,PAN Ying,WANG Xing,MA Yu-Xiang,KONG Ling-Dong.Wuling San ameliorates urate under-excretion and renal dysfunction in hyperuricemic mice[J].Chinese JOurnal of Natural Medicines,2013(3):214-221.
Authors:DING Xiao-Qin  PAN Ying  WANG Xing  MA Yu-Xiang  KONG Ling-Dong
Institution:State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210093, China
Abstract:AIM: The present study was undertaken to characterize the effects of Wuling San on urate excretion and renal function, and explore its possible mechanisms of action in hyperuricemic mice. METHODS: Mice were administered with 250 mg.kg-1 potas- sium oxonate by gavage once daily (10 animals/group) for seven consecutive days to develop a hyperuricemia model. Different doses of Wuling powder were orally initiated on the day 1 h after oxonate was given, separately. Allopurinol was used as a positive control. Serum and urine levels of uric acid and creatinine, and fractional excretion of uric acid (FEUA) were measured in hyperuricemic mice treated with Wuling San and allopurinol. Simultaneously, renal mRNA and protein levels of urate transporter 1 (mURAT1), glucose transporter 9 (mGLUT9), organic anion transporter 1 (mOAT1), as well as organic cation/carnitine transporters rnOCT1, mOCT2 and mOCTN2, were assayed by semi-quantitative RT-PCR and Western blot methods, respectively. RESULTS and CONCLUSION: Compared to the hyperuricemia control group, Wuling San significantly reduced serum uric acid and creatinine levels, increased 24 h urate and creatinine excretion, and FEUA in hyperuricemic mice, exhibiting its ability to enhance urate excretion and improve kidney function. Wuling San was found to down-regulate mRNA and protein levels of mURAT1 and mGLUT9, as well as up-regulate mOAT1 in the kidney of hyperuricemic mice. Moreover, Wuling San up-regulated renal InRNA and protein levels of mOCT1, mOCT2 and mOCTN2, leading to kidney protection in this model.
Keywords:Wuling San  Hyperuricemia  URAT1  GLUT9  OAT1  OCT1/2  OCTN2
本文献已被 维普 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号