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白鲜皮药材指纹图谱研究
引用本文:杜程芳,屠鹏飞.白鲜皮药材指纹图谱研究[J].中国天然药物,2003,1(2):94-98.
作者姓名:杜程芳  屠鹏飞
作者单位:北京大学药学院天然药物化学系,北京大学中医药现代研究中心,北京,100083
基金项目:国家“九五”重点科技攻关计划项目基金资助 (No .99 92 9 0 1 2 5 )~~
摘    要:目的:采运用反相高效液相色谱法,研究并建立白鲜皮药材的指纹图谱。方法:用Kromasil 100 C18色谱柱,乙腈-水梯度洗脱;流速1.0mL/min;检测波长228nm。结果:运用梯度洗脱得到的色谱图各色谱峰分离较好,达到指纹图谱的要求。结论:采用HPLC指纹图谱可有效控制白鲜皮的质量。

关 键 词:白鲜皮  药材  指纹图谱  中药  高效液相色谱法
文章编号:1672-3651(2003)02-0094-05
修稿时间:2003年4月26日

The HPLC/UV Fingerprint Analysis of Cortex Dictamni
DU Cheng Fang,TU Peng Fei.The HPLC/UV Fingerprint Analysis of Cortex Dictamni[J].Chinese JOurnal of Natural Medicines,2003,1(2):94-98.
Authors:DU Cheng Fang  TU Peng Fei
Institution:DU Cheng Fang,TU Peng FeiDepartment of natural medicines,School of Pharmaceutical Sciences,Peking University. Modern Research Center for Traditional Chinese Medicine,Peking University Health Science Center,Beijing 100083
Abstract:AIM:To study and establish the fingerprint of raw Cortex Dictamni by reversed phased high performance liquid chromatography. METHOD:The chromatographic conditions were as follows: a Kromasil 100 C 18 column was used; the mobile phase was composed of acetontrile and water with gradient elution;the flow rate was 1 0 ml/min and the UV absorbance detection was set at 228 nm.RESULT:Under the selected chromatographic conditions, good HPLC fingerprints of Cortex Dictamni were obtained .CONCLUSION:Quality of raw Cortex Dictamni can be controlled effectively by HPLC UV fingerprint.
Keywords:Cortex Dictamni  HPLC  Fingerprint
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