固相萃取-高效液相色谱法测定大黄汤中没食子酸、儿茶素和表儿茶素的含量(英文)

目的:建立大黄汤中没食子酸、儿茶素和表儿茶素的含量测定方法。方法:大黄汤经C18固相萃取小柱预处理后, 采用高效液相色谱法, 以梯度洗脱同时测定大黄汤中没食子酸、儿茶素和表儿茶素的含量, 选用安捷伦 Zorbax SB-C18柱(250 mm × 4.6 mm, 5 μm); 检测波长 210 nm; 柱温30 ℃; 流速1mL·min·1。结果:没食子酸、儿茶素、表儿茶素的定量限分别为1.86、2.32 和2.00 μg·mL·1, 没食子酸、儿茶素、表儿茶素分别在 4.66·233、5.80·290、2.00·100 μg·mL·1 时与峰面积线性关系良好,回收率为87.9%·103.0%, 日内及日间精密度的 RSD 分别小于0.12%、2.23%。结论:该方法简单、准确,可用于大黄药材及其制剂质量评价。

A validated solid-phase extraction HPLC method for the simultaneous determination of gallic acid, catechin and epicatechin in rhubarb decoction

AIM： To develop a new SPE-HPLC method for the simultaneous determination of gallic acid, catechin and epicatechin in rhubarb decoction. METHODS： The analytes were pretreated by solid-phase extraction using a C18 cartridge and separated on an Agilent Zorbax SB-C18 （250 mm × 4.6 mm, 5 μm） analytical column by gradient elution using a mixture of methanol （0.06% formic acid） and water （0.1% formic acid） at 30 ℃. RESULTS： The method was found to be linear in the 4.66-233, 5.80-290 and 2.00-100 μg.mL-1 concentration range for gallic acid, catechin and epicatechin （r 〉 0.999）, respectively. The overall RSD precision value （intraand inter-day） for the retention times and peak-areas were less than 0.12% and 2.23%, respectively. In addition, the recoveries from spiked control samples were determined, ranging from 87.9% to 103%. CONCLUSION： The developed method was simple, specific and accurate, and can be applied for the quality control of the raw material of rhubarb and its corresponding remedies.