| 中药款冬中肝毒吡咯里西啶生物碱的LC/MSn检测 |
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| 引用本文： | 濮社班,徐德然,张勉,周红华,王峥涛,余国奠.中药款冬中肝毒吡咯里西啶生物碱的LC/MSn检测[J].中国天然药物,2004,2(5):293-297. |
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| 作者姓名： | 濮社班 徐德然 张勉 周红华 王峥涛 余国奠 |
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| 作者单位： | 1. 中国药科大学中药学院
2. 中国药科大学分析测试中心,南京,210038 |
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| 基金项目： | 国家自然科学基金委员会 (NSFC)，国家杰出青年基金项目 (No 3 982 5 12 9)~~ |
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| 摘 要： | 目的：中药款冬中微量成分肝毒吡咯里西啶生物碱(HPA)的检测。方法：采用薄层色谱法(TLC)、液相色谱与多级质谱联用法(LC／MS^n)。结果：从不同来源的款冬药材中检测到款冬中已有报道的2种HPA，并发现款冬中还含有另外3种HPA，分子量分别为299、341和366。结论：从7份款冬药材中共检测到5种HPA，对检测到的其他种类的HPA应进一步进行提取、分离与鉴定，并研究其毒性与活性；不同来源的款冬药材含有的HPA不完全相同，应分别检测。
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| 关 键 词： | HPA TLC 中药 生物碱 MS 药材 薄层色谱法 吡咯 多级 分子量 |
| 文章编号： | 1672-3651(2004)05-0293-05 |
| 修稿时间： | 2004年1月7日 |
The Detection of Hepatotoxic Pyrrolizidine Alkaloids in Flos Farfarae by LC/MSn |
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| PU She Ban ,XU De Ran ,ZHANG Mian ,ZHOU Hong Hua ,WANG Zheng Tao ,YU Guo Dian School of Traditional Chinese Medicine, Center for Instrumental Analysis,China Pharmaceutical University,Nanjing ,China.The Detection of Hepatotoxic Pyrrolizidine Alkaloids in Flos Farfarae by LC/MSn[J].Chinese JOurnal of Natural Medicines,2004,2(5):293-297. |
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| Authors: | PU She Ban XU De Ran ZHANG Mian ZHOU Hong Hua WANG Zheng Tao YU Guo Dian School of Traditional Chinese Medicine Center for Instrumental Analysis China Pharmaceutical University Nanjing China |
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| Institution: | PU She Ban 1,XU De Ran 1,ZHANG Mian 1,ZHOU Hong Hua 2,WANG Zheng Tao 1*,YU Guo Dian 1 1School of Traditional Chinese Medicine, 2 Center for Instrumental Analysis,China Pharmaceutical University,Nanjing 210038,China |
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| Abstract: | AIM:To detect the hepatotoxic pyrrolizidine alkaloids (HPA ) in Flos Farfarae (the flower buds of Tussilago farfara L.).METHOD: The alkaloid extracts were analyzed by TLC and LC/MS n techniques.RESULT: Besides the reported 2 HPAs previously,some other HPAs,whose molecule weights (MW) were 299,341 and 366,were detected in Flos Farfarae.CONCLUSION: LC/MS n technique could detect HPA of Flos Farfarae, and more HPAs should be isolated,separated and identified from Flos Farfarae;it is also necessary to study the toxicities and activities of HPA;the types and species of HPA from various sources are different,and the PA (especial for HPA) of Flos Farfarae from different sources should be detected respectively. |
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| Keywords: | Flos Farfarae Hepatotoxic pyrrolizidine alkaloids(HPA) LC/MS n |
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