养阴益气活血方对干燥综合征NOD小鼠血清及颌下腺Th1/Th2免疫平衡的影响

目的 观察养阴益气活血方对干燥综合征（Sjogren′s Syndrome，SS）非肥胖型糖尿病（non-obese diabetic，NOD）小鼠血清及颌下腺干扰素－γ（interferon-γ， IFN-γ）、IL-2、IL-4、IL-10的表达及Th1/Th2免疫平衡的影响，探讨其作用机制。方法 32只NOD小鼠随机分为模型组、中药组［养阴益气活血方煎剂，0.4 mL（100 g/kg）］、西药组［羟氯喹0.4 mL（60 mg/kg）］、联合组［养阴益气活血方煎剂（50 g/kg）＋羟氯喹（60 mg/kg）0.4 mL］，每组8只，8只Balb/C小鼠作为正常对照（正常组）。实验进行8周后取血，摘取小鼠颌下腺组织，采用ELISA法检测小鼠血清IFN-γ、IL-2、IL-4、IL-10水平，SP法检测颌下腺组织IFN-γ、IL-2、IL-4、IL-10蛋白的表达。结果 模型组小鼠血清和颌下腺组织IFN-γ、IL-2、IL-4、IL-10水平明显高于正常组（P〈0.05）。中药组和联合组血清IFN-γ、IL-10水平均低于西药组（P〈0.05）。联合组颌下腺IFN-γ、IL-2表达低于西药组（P〈0.05）。模型组血清和颌下腺IFN-γ/IL-4比值均高于其他各组（P〈0.05），且中药组和联合组IFN-γ/IL-4比值最接近正常组。结论 养阴益气活血方能降低SS NOD小鼠血清和颌下腺Th1、Th2相关细胞因子的水平，下调血清和颌下腺IFN-γ/IL-4比值。养阴益气活血方可能通过调节SS小鼠Th1/Th2免疫平衡达到治疗作用。

Effect of Yangyin Yiqi Huoxue Recipe on Immune Balance of Th1/Th2 in Serum and Submaxillary Glands of NOD Mice with Sjogren′s Syndrome

Objective To observe the effect of Yangyin Yiqi Huoxue Recipe （YYHR） on expression of interferon-γ（ IFN-γ）, IL-2, IL-4, IL-10, and immune balance of Th1/Th2 in serum and submaxillary glands of non-obese diabetic （NOD） mice with Sjogren＇s syndrome （SS）, and to explore its mechanisms. Methods Totally 32 NOD mice were randomly into 4 groups, i.e., the model group, the Chinese medicine group CM,administered with YYHR at the dose of 0.4 mL by gavage （100 g/kg） ], the Western medicine group WM group,administered with hydroxychloroquine 0.4 mL by gavage （60 mg/kg）], and the combined group Eadministered with YYHR （50 g/kg） and hydroxychloroquine （60 mg/kg） 0.4 mL by gavage], 8 mice in each group. Eight Balb/C mice were recruited as the normal control group. Mice were sacrificed to withdraw blood after 8 weeks. The submaxillary gland was excised. Serum levels of IFN-γ,, IL- 2, IL-4, and IL-10 were detected by ELISA. Protein expression of IFN-γ, IL-2, IL-4, and IL-10 in submaxillary glands was detected by SP method. Results Compared with the normal group, levels of IFN-γ, IL-2, IL-4, and IL-10 in serum and submaxillary glands all increased in the model group （P 〈0.05）. Levels of IFN-γ, and IL-10 in serum in the CM group and the combined group were lower than those of the WM group （P 〈0.05）. Serum levels of IFN-γ, and IL-2 in submaxillary glands in combined group were lower than those of the WM group （P 〈0.05）. The ratio of IFN-γ/IL-4 in serum and submaxillary glands in the model group were higher than that of the rest groups （P 〈0.05）. Besides, it was the nearest to that of the normal group. Conclusions YYHR could decrease the levels of Thl and Th2 related cytokines and the ratio of IFN-γ/IL-4 in serum and submaxillary glands of NOD mice with SS. It could achieve therapeutic effects through adjusting immune balance of Th1/Th2 in SS mice.