子宫内膜异位症白细胞介素—8与白细胞介素—10水平及？…

目的：探讨子宫内膜异位症白细胞介素－８（ＩＬ－８）、白细胞介素－１０（ＩＬ－１０）水平及中药内异方对其调节作用。方法：根据Ｃｕｍｍｉｎｇｓ ＡＭ方法建立子宫内膜异位症动物模型，随机分成非治疗组、内异方一苯氧胺组，另设正常对照组。采用ＥＬＩＳＡ法检测ＩＬ－８、ＩＬ－１０含量。结果：非治疗组外周血及腹腔液ＩＬ－８水平、腹腔巨噬细胞数量显著高于正常对照组；内异方组异位种植内膜组织重量、腹腔巨噬细胞数量均

IL-8 and IL-10 Levels in Endometriosis and Regulated Role of Neiyifang on Them

To investigate the IL 8 and IL 10 levels in endometriosis and the regulated role of Neiyifang (NYF) on them. Methods: Animal models with endometriosis after Cummings method was established. IL 8 and IL 10 levels were determined in using double antibody sandwich ELISA. Results: The IL 8 levels of serum and peritoneal fluid, and peritoneal macrophage amounts were markedly higher in the untreated group than those in the control group. The weights of endometriotic tissue and peritoneal macrophage amounts were markedly lower in the treated group of NYF, compared with the untreated group. The lipopolysaccharide stimulated IL 10 secretion in peritoneal macrophages from the untreated group were significantly lower compared with the control group and the treated group of NYF. Conclusion: The abnormality of IL 8 and IL 10 levels in endometriosis may be related to the pathogenesis of the disease. The therapeutical mechanism of NYF was mediated by promotion of IL 10 secretion, and further restrained the composition of inflammatory media and the growth of endometriotic tissue.