IBS-D肝郁脾虚型病证症结合大鼠模型的建立与评价的初步研究

目的建立一种疾病一证候一症状相结合的腹泻型肠易激综合征（diarrhea．predominantirri．tablebowelsyndrome，IBS—D）肝郁脾虚型大鼠模型。方法（1）模型建立方法：采用新生母子分离＋慢性束缚＋番泻叶灌胃法复制IBS—D肝郁脾虚型病证症结合大鼠模型。将48只实验动物分为正常组、母子分离组、束缚组、母子分离＋束缚组、母子分离＋番泻叶组、三因素组（母子分离十束缚＋番泻叶组）6组，每组8只。（2）模型评价方法：以结直肠扩张的疼痛阈值代表内脏敏感性，评价“疾病”模型的建立；以旷场实验和血清D一木糖水平评价肝郁脾虚“证型”的建立；以排便粒数和稀便率评价腹泻“症状”的建立。结果（1）与正常组比较，三因素组大鼠的体重增长量较少，差异有统计学意义（P〈0．05）。（2）三因素组大鼠疼痛闽值明显降低，与正常组比较，差异有统计学意义（P〈0．05）。（3）与正常组比较，三因素组大鼠总穿格数、站立次数、修饰次数均明显减少，差异有统计学意义（P〈0．05）。（4）与正常组比较，三因素组大鼠血清D一木糖含量显著下降，差异有统计学意义（尸〈0．05）。（5）与正常组比较，三因素组大鼠排便粒数及稀便率差异有统计学意义（P〈0．05）。结论新生母子分离＋慢性束缚＋番泻叶灌胃的三因素复合SD模型大鼠符合IBS—D肝郁脾虚型疾病特点，可能是一种较好的研究中医药治疗IBS疗效机制的动物模型，但仍需要进一步的深入研究。

Preliminary Study of Establishing and Assessing IBS-D Model Rats of Gan Stagnation and Pi Deficiency Syndrome

Objective To establish a new disease-syndrome-symptom integrated diarrhea-pre- dominant irritable bowel syndrome （IBS-D） rat model of Gan stagnation and Pi deficiency syndrome （GSPDS）. Methods （1） The model establishment method： We combined mother-infant separation, chronic restraint, and senna gavage to establish a new IBS-D model of GSPDS. Totally 48 experimental rats were divided into the normal group （Group A）, the mother-infant separation group （Group B）, the chronic restraint group （Group C）, and the senna gavage group （Group D）, the mother-infant separation ＋ senna gavage group （Group E）, and the mother-infant separation ＋ chronic restraint ＋ sen- na gavage group （Group F）, 8 in each group. （2） The model evaluation method： We used pain threshold indicating colorectal distension to represent for the visceral sensitivity, thus evaluating the establishment of ＂disease＂ model; open field test and serum D-xylose levels to evaluate the establishment of GSPDS model; defecation numbers of grain and loose stool rate to evaluate the establishment of diarrhea symp- tom. Results （1） Compared with Group A, the body weight gained less in Group F, showing statistical difference （P 〈0.05）. （2） The pain threshold significantly decreased in Group F, showing statisticaldifference when compared with Group A （P 〈0.05）. （3） Compared with Group A, the total cross num- ber, the standing number, and the decoration number in Group F significantly decreased （P 〈0.05）. （4） Compared with Group A, the serum D-xylose level of Group F significantly decreased （P 〈0.05）. （5） Compared with Group A, the defecation numbers of grain and the loose stool rate significantly increased, showing statistical difference （P 〈 0.05 ）. Conclusions A new disease-syndrome-symptom integrated IBS-D animal model of GSPDS successfully established might be a better animal model used for studying IBS by Chinese medicine. However, further studies are needed.