补肾益智方对Alzheimer病模型大鼠脑内生长抑素及其mRNA表达阳性神经元的影响

目的：观察补肾益智方对Ｄ－半乳糖加速衰老加上脑Ｍｅｙｎｅｒｔ核损毁Ｚｌｚｈｅｒｍｅｒ病（ＡＤ）模型大鼠脑内生长抑素（ｓｏｍａｔｏｓｔａｔｉｎ，ＳＳ）的影响。方法：将１５月龄初老年雌性Ｗｉｗｔａｒ大鼠随机正常对照组、ＡＤ模型组、双益平对照组和补肾益智方观察组。模型组大鼠以Ｄ－半乳糖腹腔注射加上鹅膏到（ｉｂｏｔｎｉｃ ａｃｉｄ，ＩＢＯ）脑内Ｍｅｙｎｅｒｔ核注射造模型，双益平对照组和补佃智方观察组分别在

Effects of Bushen Yizhi Recipe on Somatostatin-like Immunopositive and Somatostatin Messenger Ribonucleic Acid Expressed-Positive Neurons in Alzheimer's Disease Model Rats

To observe the effect of Bushen Yizhi Recipe (BYD) on somatostatin-like (SS-like) immunoreactivity and SS mRNA expressed-positive neurons in Alzheimer disease (AD) model rats induced by D-galactose intraperitoneal and ibotenic acid (IBO) intracerebral injection. Methods: Fifteen months Wistar rats were randomly divided into 4 groups: normal control; AD model control induced by D-galactose intraperitoneal injection and lesion of basal nucleus of Meynert with IBO; Huperzine A (Hup-A) treated group and BYD treated group fed with Hup-A or BYD respectively. The SS-like immunoreactivity and SS mRNA expressed-positive neurons in parietal lobe, hippocampal CAI field and hilus of the dentate gyrus in the brains of rats, which were detected with immunohistochemistry and DIG-labelling cRNA-probe in situ hybridization. Results: Counts and integrations of optic density of SS-like immuno-reactivity and SS-mRNA expressed neurons in parietal lobe, hippocampal CAI field and hilus of the dentate gyrus in BYD treated group increased compared with AD model rats. Conclusion: BYD might protect the SS-ergic system against impairment of D-galactose and IBO in the brains of the AD model rats.