胰岛素抵抗模型大鼠的中医证候研究

目的：探讨胰岛素抵抗（IR）模型大鼠的中医证候。方法：用含60％蔗糖饲料诱导大鼠产生IR，对造模后有关指标的相关系数进行聚类分析。结果：IR大鼠的指标变化可分为3类，并与中医的痰浊，瘀血，内毒有密切的联系。痰浊证表现为血脂（甘油三脂，总胆固醇）升高，糖化血清蛋白增多，瘀血证表现为凝血酶原时间缩短，血浆纤维蛋白原含量升高，血液高粘状态，红细胞和血小板数升高及血压变化，内毒证主要表现为葡萄糖毒性和肿瘤坏死因子α毒性及血清胰岛素升高。结论：IR大鼠模型具有痰浊，瘀血和内毒互结的中医证候，为中医临床与实验研究提供了理论依据。

Study on Syndrome Pattern in Insulin Resistant Model Rats

To explore the Syndrome pattern in insulin resistant model rats. Methods: Eight Sprague Dawley (SD) rats were induced to insulin resistance (IR) by 60% high sucrose forage. Results: The correlative coefficient of some biological and internal changes were clusterly analyzed and divided into 3 groups, which was closely related with phlegm turbidity, blood stasis and internal toxin types respectively. The phlegm turbidity type was characterized by high content of blood lipid (triglyceride, total cholesterol) and high concentration of glycated serum protein; the blood stasis type was characterized by tendency of high viscosity and high coagulant state, the blood pressure increased, prothrombin time shortened, fibrinogen content raised and RBC and platelet count increased; while the internal toxin type was characterized by high content of glucose, insulin and the elevation of tumor necrosis factor. Conclusion: The combined Syndrome of phlegm turbidity, blood stasis and internal toxin is the Syndrome pattern in insulin resistant model rat. This observation provides theoretic basis for clinical and experimental studies of TCM.