银杏叶提取物金纳多心停跳液对人心肌细胞bcl-2和bcl-xL基因表达的影响

目的探讨银杏叶提取物金纳多（简称金纳多）对体外循环人心肌细胞抗凋亡基因bcl-2和bcl—xL表达的影响。方法择期先天性心脏病患儿30例，随机均分为两组，治疗组在灌注St Thomas’停搏液（4℃）中加入金纳多（0．5mg／kg），心脏完全停跳后开始心内手术；对照组单纯灌注StThomas’停搏液（4℃）。在手术结束开放升主动脉前取心肌组织，采用RT—PCR技术在凝胶电泳成像基础上半定量检测bcl-2和bcl—xL基因的表达水平。结果治疗组bcl-2和bcl—xL基因的表达显著高于对照组（P〈0．05）。结论银杏叶提取物金纳多可促进人心肌细胞抗凋亡基因bel-2和bel—xL的表达。

Effects of Ginkgo biloba Extract (Ginaton) on mRNA Expression of bcl-2 and bcl-xL in Myocardium of Patients Underwent Hypothermic Cardiopulmonary Bypass

OBJECTIVE: To investigate the effects of Ginkgo biloba extract (Ginaton) on bcl-2 and bcl-xL mRNA expression in the myocardium of patients underwent hypothermic cardiopulmonary bypass (CPB). METHODS: Thirty congenital heart disease patients were randomly assigned to 2 groups, the control group and the treated group. Patients in both groups received St. Thomas' cardioplegic solution via radix aortae, while Ginaton (0.5 mg/kg) was added in the treated group. Cardiac surgery was started after complete heart arrest. Myocardium was taken before the aorta ascendens was unblocked and mRNA expression of bcl-2 and bcl-xL in the ventricular tissue was detected by RT-PCR. RESULTS: The gene expressions of bcl-2 and bcl-xL were significantly higher in the treated group than those in the control group (P < 0.05). CONCLUSION: Ginaton could promote the mRNA expressions of the antiapoptotic gene bcl-2 and bcl-xL in myocardium of patients underwent CI'PB.