针刺改善大鼠胚泡着床障碍机制初探

目的 观察针刺对胚泡着床障碍大鼠胚泡着床的影响，并初步探讨其作用机制.方法 早孕大鼠随机分为正常组、模型组及针刺组，观察各组大鼠着床率及平均着床胚泡数；放免法检测血清雌二醇（E2）、孕酮（P4）及泌乳素（PRL）的水平；免疫组化及RT—PCR法检测着床点孕激素受体（PR）及泌乳素受体（PRLR）表达的差异。结果 针刺组着床率及平均着床胚泡数较模型组显著提高（P〈0．01），模型组血清P4、PRL水平、PR、PRLR蛋白及mRNA表达水平均低于正常组和针刺组（P〈0．05）。结论 针刺可在一定程度上促进大鼠胚泡着床，其作用机制可能与针刺调节大鼠体内性激素及其受体表达有关，

Preliminary Study on the Mechanisms of Acupuncture in Promoting Embryo Implantation in Rats

Objective To observe the influence of acupuncture on embryo implantation in rat model of embryo implantation dysfunction,and to primarily explore its possible mechanisms.Methods Pregnant rats were randomly allocated into the control group,the model group and the acupuncture group.The pregnancy rate and average number of blastocyst were observed,the serum levels of estrodiol(E2),progesterone(P4)and prolactin(PRL)were detected by RIA,and the protein and mRNA expression of progesterone receptor(PR)and prolactin receptor(PRLR)in endometrial tissue of implantation site were determined using immunohistochemical assay and RT-PCR respectively.Results The pregnancy rate and average number of blastocyst were significantly higher in the acupuncture group than those in the control group respectively(P<0.01).The serum levels of P4 and PRL as well as the protein and mRNA expression levels of PR and PRLR in the model group were significantly lower than those in the other two groups(P<0.05).Conclusion Acupuncture can promote embryo implantation in rats to a certain degree,and its mechanism might be related with the effects of acupuncture in mediating the sexual hormone levels and the receptor expression of rats.