清开灵抗大鼠谷氨酸神经毒性脑损伤的实验研究

目的：观察清开灵对大鼠谷氨酸神经毒性脑损伤的作用。以寻找有效的抗谷氨酸神经毒性的中西药物。方法：采用细胞形态计量、电镜及免疫组织化学方法，观察清开灵对谷氨酸所致大鼠脑损伤的影响。结果：清开灵可对抗谷氨酸所致的大鼠脑组织水、钠含量的增加，减轻谷氨酸所致的皮层及海马细胞形态结构的损伤，降低脑组织中ｃ－ｆｏｓ阳性细胞率。结论：清开灵具有抗谷氨酸神经毒性作用。其作用机制与抑制谷氨酸所致的ｃ－ｆｏｓ基因表达

Experimental Study of Protective Effect of Qingkailing on Glutamate Induced Neurotoxic Damage of Brain

Objective: To observe the effect of Qingkailing (QKL) on glutamate induced damage of brain, in order to seek for effective drugs for antagonizing neurotoxicity of glutamate. Methods: Number and morphology metrology of neuron in cerebral cortex and hippocampus were detected by using MIAS 300 image analysing instrument, electron microscope and immunohistochemical methods. Results: QKL could alleviate the glutamate induced accumulation of water and sodium in the brain tissue, relief the damage of cerebral cells and reduce the percentage of c fos positive cell in brain. Conclusion: QKL could protect brain damage induced by glutamate, which might be related to inhibition of QKL on glutamate caused enhancement of c fos gene expression.