黄芪提取液对犬离体肾低温灌注与保存影响的实验研究

目的：研究黄芪提取液对犬离体肾低温灌注及保存中缺血再灌注损伤的影响。方法：以常规高渗枸橼酸盐腺嘌呤液(HC-A液)为对照组，以HC-A液添加黄芪提取液为实验组，分别对离体犬肾进行低温灌注保存，通过光镜、电镜观察肾组织结构变化，并建立犬肾移植模型，检测移植前后生化指标变化，并进行综合分析。结果：实验组保存的犬肾组织超微结构损伤明显轻于对照组。移植肾早期功能指标检测，实验组血肌酐值明显低于对照组，而内生肌酐清除值明显高于对照组，两组比较，差异均具有显著性(P＜0．05)。结论：黄芪提取液用于肾脏低温灌注保存中，对缺血再灌注损伤的肾脏有一定保护作用。

Experimental Study on Effect of Astragalus Extractum on Canine Isolated Kidney during Hypothermia Perfusion and Preservation

Objective:To investigate the effect of Astragalus Extractum on canine isolated kidney during hypothermia perfusion and preservation. Methods:Isolated kidneys in the control group were hypothermia perfused and preserved using conventional hypertonic adenine citrate solution (HC-A), and for those in the experimental group, using HC-A plus Astragalus extract instead. The changes of renal tissue construction were observed with light microscopy and electron microscopy. Moreover, the kidney transplantation model of dog was established to determine the changes of biochemical parameters before and after transplantation. Data were analysed synthetically. Results:The ultrasturctural injury in preserved kidney of the experimental group was significantly milder than that in the control group. Parameters determined in the early stage of transplantation showed that the blood creatinine level was significantly lower and the endogenous creatinine clearing value was higher in the experimental group than that in the control group, the difference was significant (P<0.05). Conclusion:When Astragalus extractum is used in preserving kidney with hypothermia perfusion, it shows definite protective effect on the ischemic reperfusion injured kidney.