活血药对人肺癌细胞粘附和侵袭的影响

目的：观察活血药川芎嗪，水蛭素，丹参酮ⅡＡ和凝血酶对高转移人巨细胞肺癌ＰＧＣＬ３和低转移人肺腺癌ＰＡａ细胞的粘附和侵袭的影响。方法；用不同浓度的川芎嗪，丹参酮ⅡＡ，水蛭素和凝血酶分别处理ＰＧＣＬ３和ＰＡａ和细胞，观察了ＰＧＣＬ３和ＰＡａ细胞单独或与血小板作用后对纤维粘连蛋白的粘附和Ｂｏｙｄｅｎ小室的侵袭改变。结果：川芎嗪，水蛭素和凝血酶在不同程度上增加ＰＧＣＬ３细胞的粘附，而丹参酮ⅡＡ抑制这种粘附

Influence of Blood Activating Drugs on Adhesion and Invasion of Cells in Lung Cancer Patients

OBJECTIVE: To investigate the influence of some blood-activating drugs like Tetramethylpyrazine, Tanshinone II A, Hirudin and thrombin on adhesive and invasive behavior of PGCL3 and PAa cell lines. METHODS: Using the above-mentioned blood-activating drugs in various concentration to treat PGCL3 and PAa cells, and the changes in adhesion to fibronectin and invasion in Boyden Chamber of these cells, alone or after interacted with human platelets, were observed. RESULTS: Tetramethylpyrazine, Hirudin and thrombin could increase the adhesion of cells to fibronectin and Tanshinone II A decrease it. Tetramethylpyrazine, Tanshinone II A and Hirudin inhibited the invasion of PGCL3 cells in Boyden Chamber, and thrombin augmented the process. CONCLUSION: The blood-activating drugs may either inhibit or promote the invasion and metastasis of PGCL3 and PAa cells in the light of various conditions.